Gene expression changes in mononuclear cells in patients with metabolic syndrome after acute intake of phenol-rich virgin olive oil
Camargo A, Ruano J, Fernandez JM, Parnell LD, Jimenez A, Santos-Gonzalez M, Marin C, Perez-Martinez P, Uceda M, Lopez-Miranda J, Perez-Jimenez F, Perez-Jimenez F
BMC genomics · 106 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Randomized controlled trial (indexed by PubMed)
- Studied in
- People
- Main outcome
- Mechanisms only
- Intake measured by
- Food provided by researchers
Who paid for it
- Funding
- Independent funding
- University or hospital
- Centro de Investigación Biomédica en Red-Fisiopatología de la Obesidad y Nutrición
- Government
- Ministerio de Medio Ambiente y Medio Rural y Marino
- Government
- Instituto de Salud Carlos III
Based on 3 listed funder(s).
Publication
- Published
- 2010-04-20 · BMC Genomics · vol. 11 · issue 1 · p. 253
- Publisher
- BioMed Central
- Cited
- 156 citations · more than 98% of similar papers · 7.9× the field average
- Impact
- Top 10% most cited in its field
- References
- 53 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Edible Oils Quality and Analysis · Fatty Acid Research and Health · Phytochemicals and Antioxidant Activities
- Keywords
- Biology, Peripheral blood mononuclear cell, Gene expression, Olive oil, Gene, DNA microarray, Metabolic syndrome, Food science, Molecular biology, Biotechnology, Genetics, Endocrinology, Obesity, In vitro
- MeSH
- leukocytes, mononuclear, humans, phenols, dietary fats, plant oils, oligonucleotide array sequence analysis, gene expression profiling, postprandial period, gene regulatory networks, metabolic networks and pathways, olive oil, metabolic syndrome
11 authors
From ES, US
- Antonio CamargoInstituto Maimónides de Investigación Biomédica de Córdoba; Centro de Investigación Biomédica en Red; University of Córdoba
- Juan A. RuanoInstituto Maimónides de Investigación Biomédica de Córdoba; Centro de Investigación Biomédica en Red; University of Córdoba
- Juan Marcelo FernándezInstituto Maimónides de Investigación Biomédica de Córdoba; Centro de Investigación Biomédica en Red; University of Córdoba
- Laurence D. ParnellTufts University; Jean Mayer Human Nutrition Research Center on Aging
- Anabel JiménezInstituto Maimónides de Investigación Biomédica de Córdoba; Centro de Investigación Biomédica en Red; University of Córdoba
- Mónica Santos GonzálezUniversity of Córdoba
Abstract
Background
Previous studies have shown that acute intake of high-phenol virgin olive oil reduces pro-inflammatory, pro-oxidant and pro-thrombotic markers compared with low phenols virgin olive oil, but it still remains unclear whether effects attributed to its phenolic fraction are exerted at transcriptional level in vivo. To achieve this goal, we aimed at identifying expression changes in genes which could be mediated by virgin olive oil phenol compounds in the human.
Results
Postprandial gene expression microarray analysis was performed on peripheral blood mononuclear cells during postprandial period. Two virgin olive oil-based breakfasts with high (398 ppm) and low (70 ppm) content of phenolic compounds were administered to 20 patients suffering from metabolic syndrome following a double-blinded, randomized, crossover design. To eliminate the potential effect that might exist in their usual dietary habits, all subjects followed a similar low-fat, carbohydrate rich diet during the study period. Microarray analysis identified 98 differentially expressed genes (79 underexpressed and 19 overexpressed) when comparing the intake of phenol-rich olive oil with low-phenol olive oil. Many of these genes seem linked to obesity, dyslipemia and type 2 diabetes mellitus. Among these, several genes seem involved in inflammatory processes mediated by transcription factor NF-kappaB, activator protein-1 transcription factor complex AP-1, cytokines, mitogen-activated protein kinases MAPKs or arachidonic acid pathways.
Conclusion
This study shows that intake of virgin olive oil based breakfast, which is rich in phenol compounds is able to repress in vivo expression of several pro-inflammatory genes, thereby switching activity of peripheral blood mononuclear cells to a less deleterious inflammatory profile. These results provide at least a partial molecular basis for reduced risk of cardiovascular disease observed in Mediterranean countries, where virgin olive oil represents a main source of dietary fat. Admittedly, other lifestyle factors are also likely to contribute to lowered risk of cardiovascular disease in this region.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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