Blueberry anthocyanins and pyruvic acid adducts: anticancer properties in breast cancer cell lines
Faria A, Pestana D, Teixeira D, de Freitas V, Mateus N, Calhau C
Phytotherapy research : PTR · 67 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Funding not disclosed
Publication
- Published
- 2010-06-17 · Phytother Res · vol. 24 · issue 12 · pp. 1862–1869
- Publisher
- Wiley
- Cited
- 147 citations · more than 82% of similar papers · 1.7× the field average
- References
- 34 works
- Access
- Open access (repository copy) · OTHER-OA
- Research areas
- Phytochemicals and Antioxidant Activities · Hops Chemistry and Applications · Plant Gene Expression Analysis
- Keywords
- Anthocyanin, Pyruvic acid, Chemistry, Biochemistry, Apoptosis, Cell growth, Cell culture, Biology, Food science
- MeSH
- cell line, tumor, humans, fruit, breast neoplasms, pyruvic acid, anthocyanins, plant extracts, antineoplastic agents, phytogenic, cell proliferation, cell survival, caspase 3, blueberry plants
6 authors
From PT
- Ana Faria · correspondingUniversidade do Porto
- Diogo PestanaUniversidade do Porto
- Diana TeixeiraUniversidade do Porto
- Victor Armando Pereira de FreitasUniversidade do Porto
- Nuno Filipe da Cruz Batista MateusUniversidade do Porto
- Conceição CalhauUniversidade do Porto
Abstract
The purpose of this study was to investigate the anticancer properties of an anthocyanin-pyruvic acid adduct extract, which is being developed aiming to be further applied in the food industry. An anthocyanin extract from blueberry (extract I) and an anthocyanin-pyruvic acid adduct extract (extract II) were tested on two breast cancer cell lines (MDA-MB-231 and MCF7). Proliferation was assessed by SRB assay and ³H-thymidine incorporation. Caspase-3 activity was determined in the presence of both extracts. Their capacity as chemoattractants and their invasive potential were also assayed. In both cell lines, extracts I and II significantly reduced cell proliferation at 250 μg/mL, after 24 h of cell incubation. Caspase-3 activity was not altered by the extracts (250 μg/mL) in either cell line, with the exception of extract II in MCF-7, which increased its activity, probably explaining its effects on cell proliferation. Both extracts (250 μg/mL) demonstrated significant antiinvasive potential in both cell lines. Furthermore, they did not demonstrate any capacity for chemotaxis. In conclusion, blueberry anthocyanins and the respective anthocyanin-pyruvic acid adducts demonstrated anticancer properties by inhibiting cancer cell proliferation and by acting as cell antiinvasive factors and chemoinhibitors. The anthocyanin-pyruvic acid adduct extract showed a more pronounced effect in MDA-MB-231, suggesting an effect independent of estrogen receptors.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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