Prediction of extra virgin olive oil varieties through their phenolic profile. Potential cytotoxic activity against human breast cancer cells
Lozano-Sánchez J, Segura-Carretero A, Menendez JA, Oliveras-Ferraros C, Cerretani L, Fernández-Gutiérrez A
Journal of agricultural and food chemistry · 56 citations
Review labels
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How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Funding not disclosed
Publication
- Published
- 2010-08-26 · J Agric Food Chem · vol. 58 · issue 18 · pp. 9942–9955
- Publisher
- American Chemical Society
- Cited
- 91 citations · more than 95% of similar papers · 4.4× the field average
- Impact
- Top 10% most cited in its field
- References
- 34 works
- Access
- Paywalled
- Research areas
- Edible Oils Quality and Analysis · Cholesterol and Lipid Metabolism · Essential Oils and Antimicrobial Activity
- Keywords
- Viability assay, Chemistry, MTT assay, SKBR3, Olive oil, Cytotoxic T cell, Cancer cell, Human breast, Chromatography, Electrospray ionization, Food science, Cancer, Biochemistry, Mass spectrometry, Cell, In vitro, Biology
- MeSH
- cell line, tumor, humans, olea, fruit, breast neoplasms, phenols, plant oils, plant extracts, antineoplastic agents, phytogenic, female, olive oil
6 authors
From ES, IT
- Jesús Lozano‐SánchezUniversidad de Granada
- Antonio Segura‐Carretero · correspondingUniversidad de Granada
- Javier A. MenéndezInstitut Català d'Oncologia; Institut d'Investigació Biomèdica de Girona
- Cristina Oliveras‐FerrarosInstitut Català d'Oncologia; Institut d'Investigació Biomèdica de Girona
- Lorenzo CerretaniUniversity of Bologna
- Alberto Fernández‐GutiérrezUniversidad de Granada
Abstract
The aim of this work was to develop a rapid resolution liquid chromatography coupled to electrospray ionization time-of-flight mass spectrometry (RRLC-ESI-TOF-MS) method followed by tetrazolium salt (MTT)-based cell viability assays for qualitative and quantitative classification of extra virgin olive oil (EVOO) varieties by phenolic and other polar compound contents as well as for rapid characterization of putative cytotoxic activities against human cancer cells. Five different Spanish EVOO varieties were analyzed, and RRLC-ESI-TOF-MS method was applied for qualitative and quantitative identification of most important phenolic compounds. We finally employed MTT-based cell viability protocol to assess the effects of crude EVOO phenolic extracts (PEs) on the metabolic status of cultured SKBR3 human breast cancer cells. MTT-based cell viability assays revealed a wide range of breast cancer cytotoxic potencies among individual crude PE obtained from EVOO monovarietals. Remarkably, breast cancer cell sensitivity to crude EVOO-PEs was up to 12 times higher in secoiridoids enriched-PE than in secoiridoids-low/null EVOO-PE.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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