Coffee, broccoli and spices are strong inducers of electrophile response element-dependent transcription in vitro and in vivo - studies in electrophile response element transgenic mice
Balstad TR, Carlsen H, Myhrstad MC, Kolberg M, Reiersen H, Gilen L, Ebihara K, Paur I, Blomhoff R
Molecular nutrition & food research · 60 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Animal study (classified by our AI screen)
- Studied in
- People, plus animal or lab work
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Funding not disclosed
Publication
- Published
- 2010-09-08 · Mol Nutr Food Res · vol. 55 · issue 2 · pp. 185–197
- Publisher
- Wiley
- Cited
- 82 citations · more than 94% of similar papers · 3.7× the field average
- Impact
- Top 10% most cited in its field
- References
- 59 works
- Access
- Paywalled
- Research areas
- Genomics, phytochemicals, and oxidative stress · Redox biology and oxidative stress · Sirtuins and Resveratrol in Medicine
- Keywords
- Luciferase, Curcumin, In vivo, Transcription factor, Biochemistry, Antioxidant, Response element, Transgene, Promoter, Biology, Pharmacology, Chemistry, Gene expression, Gene, Transfection
- MeSH
- cells, cultured, animals, mice, transgenic, humans, mice, brassica, flowering tops, curcumin, quinidine, rna, messenger, plant extracts, diet, transcription, genetic, genes, reporter, coffee, spices, female, male, nf-e2-related factor 2, promoter regions, genetic, hep g2 cells, abietanes
9 authors
From NO
- Trude Rakel BalstadUniversity of Oslo
- Harald CarlsenUniversity of Oslo
- Mari C. W. MyhrstadUniversity of Oslo
- Marit KolbergUniversity of Oslo
- Hanne ReiersenUniversity of Oslo
- Lene GilenUniversity of Oslo
Abstract
Scope
Cytoprotective gene products, e.g. phase II - and antioxidant enzymes, are important in cellular redox homeostasis. A common feature of these genes is binding sites for transcription factor nuclear factor erythroid-2-related factor 2 (Nrf2), named electrophile response elements (EpREs) within their promoters.
Methods and results
To identify dietary bioactive compounds and foods with Nrf2/EpRE inducing properties in an intact organism, we utilized transgenic mice encoding luciferase under control of EpRE from the thioredoxin promoter. We found that 18 of 31 phytochemicals and 10 of 14 dietary plant extracts induced EpRE activity in liver HepG2 cells. Surprisingly, some dietary plant extracts showed profound inducing capability as compared to pure compounds indicating combinatorial effects of compounds found in whole foods. Furthermore, intraperitoneal injections of carnosol, curcumin and tert benzohydroquinine induced EpRE-dependent promoter activity in transgenic mice. In further experiments with curcumin, we found highly induced EpRE activity in intestine, liver, kidney and spleen. Finally, a combination extract made of coffee, thyme, broccoli, rosemary, turmeric and red onion fed orally, induced EpRE mediated luciferase in lung and adipose tissue.
Conclusion
These results show that plant-based foods contain compounds that can be absorbed and induce the antioxidant defence in a living organism in an organ-specific manner.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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