Sulforaphane synergizes with quercetin to inhibit self-renewal capacity of pancreatic cancer stem cells
Srivastava RK, Tang SN, Zhu W, Meeker D, Shankar S
Frontiers in bioscience (Elite edition) · 88 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- Kansas Bioscience Authority
- Government
- National Institutes of Health
- Government
- National Cancer Institute
- Government
- NCI NIH HHS
- Grants
- National Cancer Institute (R01CA125262-02S1); National Cancer Institute (R01CA114469); National Cancer Institute (R01CA125262); National Institutes of Health (RO1CA114469); National Institutes of Health (R01CA125262)
Based on 4 listed funder(s).
Publication
- Published
- 2010-11-28 · Front Biosci (Elite Ed) · vol. E3 · issue 2 · pp. 515–528
- Publisher
- Frontiers Media
- Cited
- 125 citations · more than 94% of similar papers · 3.9× the field average
- Impact
- Top 10% most cited in its field
- References
- 128 works
- Access
- Free to read
- Research areas
- Genomics, phytochemicals, and oxidative stress · Cancer Cells and Metastasis · Curcumin's Biomedical Applications
- Keywords
- Sulforaphane, Cancer stem cell, Pancreatic cancer, Cancer research, Stem cell, Chemistry, Homeobox protein NANOG, XIAP, Cell biology, Metastasis, Cancer, Apoptosis, Biology, Biochemistry, Caspase, Embryonic stem cell, Programmed cell death, Induced pluripotent stem cell
- MeSH
- cell line, tumor, humans, lentivirus, pancreatic neoplasms, thiocyanates, isothiocyanates, sulfoxides, quercetin, homeodomain proteins, proto-oncogene proteins c-bcl-2, blotting, western, analysis of variance, apoptosis, drug synergism, genetic vectors, x-linked inhibitor of apoptosis protein, neoplastic stem cells, nanog homeobox protein
1 author
From US
- Rakesh K. Srivastava · correspondingThe University of Kansas Cancer Center
Abstract
According to the cancer stem cell hypothesis, the aggressive growth and early metastasis of cancer may arise through dysregulation of self-renewal of stem cells. The objectives of this study were to examine the molecular mechanisms by which sulforaphane (SFN, an active compound in cruciferous vegetables) inhibits self-renewal capacity of pancreatic cancer stem cells (CSCs), and synergizes with quercetin, a major polyphenol and flavonoid commonly detected in many fruits and vegetables. Our data demonstrated that SFN inhibited self-renewal capacity of pancreatic CSCs. Inhibition of Nanog by lentiviral-mediated shRNA expression enhanced the inhibitory effects of sulforaphane on self-renewal capacity of CSCs. SFN induced apoptosis by inhibiting the expression of Bcl-2 and XIAP, phosphorylation of FKHR, and activating caspase-3. Moreover, SFN inhibited expression of proteins involved in the epithelial-mesenchymal transition (beta-catenin, vimentin, twist-1, and ZEB1), suggesting the blockade of signaling involved in early metastasis. Furthermore, the combination of quercetin with SFN had synergistic effects on self-renewal capacity of pancreatic CSCs. These data suggest that SFN either alone or in combination with quercetin can eliminate cancer stem cell-characteristics.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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