Unusual pungency from extra-virgin olive oil is attributable to restricted spatial expression of the receptor of oleocanthal
Peyrot des Gachons C, Uchida K, Bryant B, Shima A, Sperry JB, Dankulich-Nagrudny L, Tominaga M, Smith AB, Beauchamp GK, Breslin PA
The Journal of neuroscience : the official journal of the Society for Neuroscience · 92 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- People, plus animal or lab work
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- National Science Foundation
- University or hospital
- Harvard University
- Government
- National Institutes of Health
- Government
- National Cancer Institute
- Government
- National Institute on Deafness and Other Communication Disorders
- Government
- National Institute of General Medical Sciences
- Government
- NIGMS NIH HHS
- Government
- NIDCD NIH HHS
- Government
- NCI NIH HHS
- Grants
- National Institute on Deafness and Other Communication Disorders (P50-DC006760); National Institute on Deafness and Other Communication Disorders (R01DC002995); National Institute of General Medical Sciences (GM-29028); National Institute of General Medical Sciences (R01 GM029028); National Institute on Deafness and Other Communication Disorders (DC 02995); National Institute on Deafness and Other Communication Disorders (R29 DC002995); National Science Foundation (0216310); National Cancer Institute (F32 CA121716); National Institute on Deafness and Other Communication Disorders (DC006760); National Institutes of Health (DC02995)
Based on 9 listed funder(s).
Publication
- Published
- 2011-01-19 · J Neurosci · vol. 31 · issue 3 · pp. 999–1009
- Publisher
- Society for Neuroscience
- Cited
- 141 citations · more than 98% of similar papers · 5.9× the field average
- Impact
- Top 10% most cited in its field
- References
- 47 works
- Access
- Open access (hybrid journal) · CC-BY-NC-SA
- Research areas
- Ion Channels and Receptors · Biochemical Analysis and Sensing Techniques · Neurobiology and Insect Physiology Research
- Keywords
- Transient receptor potential channel, Chemistry, Sensory system, Pungency, Irritation, TRPV1, Neuroscience, Receptor, Pharmacology, Biology, Biochemistry, Immunology
- MeSH
- pharynx, neurons, cells, cultured, humans, aldehydes, phenols, plant oils, calcium channels, nerve tissue proteins, immunohistochemistry, adult, transient receptor potential channels, olfactory perception, hek293 cells, olive oil, trpa1 cation channel, cyclopentane monoterpenes
10 authors
From US, JP
- Catherine Peyrot des GachonsMonell Chemical Senses Center
- Kunitoshi UchidaThe Graduate University for Advanced Studies, SOKENDAI; Okazaki Institute for Integrative Bioscience; National Institute for Physiological Sciences
- Bruce P. BryantMonell Chemical Senses Center
- Asako ShimaOkazaki Institute for Integrative Bioscience
- Jeffrey B. SperryUniversity of Pennsylvania
- Luba Dankulich‐NagrudnyMonell Chemical Senses Center
Abstract
Oleocanthal, a major phenolic compound in extra-virgin olive oil with antiinflammatory properties, elicits an unusual oral pungency sensed almost exclusively in the throat. This contrasts with most other common oral irritants, such as cinnamaldehyde, capsaicin, and alcohol, which irritate mucus membranes throughout the oral cavity. Here, we show that this rare irritation pattern is a consequence of both the specificity of oleocanthal for a single sensory receptor and the anatomical restriction of this sensory receptor to the pharynx, within the oral cavity. We demonstrate, in vitro, that oleocanthal selectively activates the hTRPA1 channel in HEK 293 cells and that its ability to excite the trigeminal nervous system in rodents requires a functional TRPA1. Moreover, we similarly demonstrate that the over-the-counter analgesic, ibuprofen, which elicits the same restricted pharyngeal irritation as oleocanthal, also specifically excites rodent sensory neurons via TRPA1. Using human sensory psychophysical studies and immunohistochemical TRPA1 analyses of human oral and nasal tissues, we observe an overlap of the anatomical distribution of TRPA1 and the regions irritated by oleocanthal in humans. These results suggest that a TRPA1 (ANKTM1) gene product mediates the tissue sensitivity to oleocanthal within the oral cavity. Furthermore, we demonstrate that, despite the fact that oleocanthal possesses the classic electrophilic reactivity of many TRPA1 agonists, it does not use the previously identified activation mechanism via covalent cysteine modification. These findings provide an anatomical and molecular explanation for a distinct oral sensation that is elicited by oleocanthal and ibuprofen and that is commonly experienced around the world when consuming many extra-virgin olive oils.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY-NC-SA).
Community trust
Loading…
How much do you trust this study's findings?
Comments
Sign in to rate, comment on or flag this study.Sign inSomething wrong here?
Flag this study if its information, labels or funding look wrong. An editor reviews every flag.
Sign in to rate, comment on or flag this study.Sign inEducational information about published research. Not medical advice, and not a recommendation to start or stop anything.