Cruciferous vegetable supplementation in a controlled diet study alters the serum peptidome in a GSTM1-genotype dependent manner
Brauer HA, Libby TE, Mitchell BL, Li L, Chen C, Randolph TW, Yasui YY, Lampe JW, Lampe PD
Nutrition journal · 25 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Randomized controlled trial (indexed by PubMed)
- Studied in
- People
- Main outcome
- Mechanisms only
- Intake measured by
- Food provided by researchers
Who paid for it
- Funding
- Independent funding
- Government
- National Institutes of Health
- Government
- National Cancer Institute
- Government
- NCI NIH HHS
- Grants
- National Cancer Institute (R01 CA 92288); National Cancer Institute (R01CA070913); National Cancer Institute (F31 CA126471); National Cancer Institute (R01CA126205); National Cancer Institute (R56 CA070913)
Based on 3 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2011-01-27 · Nutr J · vol. 10 · issue 1 · p. 11
- Publisher
- BioMed Central
- Cited
- 33 citations · more than 88% of similar papers · 2.2× the field average
- References
- 36 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Glutathione Transferases and Polymorphisms · Genomics, phytochemicals, and oxidative stress · Epigenetics and DNA Methylation
- Keywords
- Cruciferous vegetables, Sulforaphane, Clinical nutrition, Genotype, Food science, Medicine, Crossover study, Internal medicine, Biology, Biochemistry, Cancer, Gene
- MeSH
- humans, brassicaceae, vegetables, isothiocyanates, glutathione transferase, peptides, prealbumin, blood proteins, diet, linear models, risk factors, cross-over studies, genotype, adult, female, male, young adult, alpha-2-hs-glycoprotein
9 authors
From US, CA
- Heather Ann BrauerUniversity of Washington
- Tanya LibbyFred Hutch Cancer Center
- Breeana L. MitchellUniversity of Washington; Fred Hutch Cancer Center
- Lin LiFred Hutch Cancer Center
- Chu ChenUniversity of Washington; Fred Hutch Cancer Center
- Timothy W. RandolphFred Hutch Cancer Center
Abstract
Background
Cruciferous vegetable intake is inversely associated with the risk of several cancers. Isothiocyanates (ITC) are hypothesized to be the major bioactive constituents contributing to these cancer-preventive effects. The polymorphic glutathione-S-transferase (GST) gene family encodes several enzymes which catalyze ITC degradation in vivo.
Methods
We utilized high throughput proteomics methods to examine how human serum peptides (the "peptidome") change in response to cruciferous vegetable feeding in individuals of different GSTM1 genotypes. In two randomized, crossover, controlled feeding studies (EAT and 2EAT) participants consumed a fruit- and vegetable-free basal diet and the basal diet supplemented with cruciferous vegetables. Serum samples collected at the end of the feeding period were fractionated and matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry spectra were obtained. Peak identification/alignment computer algorithms and mixed effects models were used to analyze the data.
Results
After analysis of spectra from EAT participants, 24 distinct peaks showed statistically significant differences associated with cruciferous vegetable intake. Twenty of these peaks were driven by their GSTM1 genotype (i.e., GSTM1+ or GSTM1- null). When data from EAT and 2EAT participants were compared by joint processing of spectra to align a common set, 6 peaks showed consistent changes in both studies in a genotype-dependent manner. The peaks at 6700 m/z and 9565 m/z were identified as an isoform of transthyretin (TTR) and a fragment of zinc α2-glycoprotein (ZAG), respectively.
Conclusions
Cruciferous vegetable intake in GSTM1+ individuals led to changes in circulating levels of several peptides/proteins, including TTR and a fragment of ZAG. TTR is a known marker of nutritional status and ZAG is an adipokine that plays a role in lipid mobilization. The results of this study present evidence that the GSTM1-genotype modulates the physiological response to cruciferous vegetable intake.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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