Chemopreventive effects of synthetic C-substituted diindolylmethanes originating from cruciferous vegetables in human oral cancer cells
Shin JA, Shim JH, Choi ES, Leem DH, Kwon KH, Lee SO, Safe S, Cho NP, Cho SD
European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP) · 12 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- University or hospital
- Kyungpook National University
- Government
- National Research Foundation of Korea
Based on 2 listed funder(s).
Publication
- Published
- 2011-05-19 · Eur J Cancer Prev · vol. 20 · issue 5 · pp. 417–425
- Publisher
- Lippincott Williams & Wilkins
- Cited
- 15 citations · more than 74% of similar papers · 0.9× the field average
- References
- 0 works
- Access
- Paywalled
- Research areas
- Genomics, phytochemicals, and oxidative stress · Nitrogen and Sulfur Effects on Brassica · Synthesis and Biological Evaluation
- Keywords
- Apoptosis, Endoplasmic reticulum, Programmed cell death, Cancer cell, Cruciferous vegetables, Cytochrome c, Chemistry, Caspase, Cell biology, Cancer research, Caspase 3, Biology, Molecular biology, Cancer, Biochemistry, Genetics
- MeSH
- tumor cells, cultured, endoplasmic reticulum, humans, vegetables, mouth neoplasms, cinnamates, thiourea, indoles, poly(adp-ribose) polymerases, ppar gamma, anticarcinogenic agents, blotting, western, apoptosis, cell proliferation, bcl-2-associated x protein, transcription factor chop, caspase 8, receptors, tnf-related apoptosis-inducing ligand, membrane potential, mitochondrial
9 authors
From KR, US
- Ji-Ae ShinJeonbuk National University
- Jung‐Hyun Shim
- Eun-Sun Choi
- Dae‐Ho LeemJeonbuk National University
- Ki Han KwonGwangju University
- Syng‐Ook LeeTexas A&M Health Science Center; Texas A&M University
Abstract
Diindolylmethane (DIM), an isothiocyanate found in cruciferous vegetables, has been shown to have cancer chemopreventive effects. A series of synthetic C-substituted DIMs (C-DIMs) analogs was developed, including DIM-C-pPhtBu and DIM-C-pPhC6H5, which exhibited better inhibitory activity in cancer cells than DIM. This study examined the effects of C-DIMs on the growth of human oral cancer cells. DIM-C-pPhtBu and DIM-C-pPhC6H5 decreased the number of viable KB cells and induced caspase-dependent apoptosis. The apoptotic cell death was accompanied by a change in Bax/Bcl-2 ratio and damage to mitochondrial membrane potential through the induction of death receptor 5 and the cleavage of Bid and caspase 8. Studies on the mechanism of action showed that the apoptotic cell death induced by DIM-C-pPhtBu and DIM-C-pPhC6H5 was mediated by endoplasmic reticulum stress. In addition, C-DIMs inhibited cell proliferation and induced PARP cleavage through death receptor 5 and CHOP in HEp-2 and HN22 cells. This provides the first evidence that synthetic C-DIMs originating from cruciferous vegetables induce apoptosis in human oral cancer cells through the endoplasmic reticulum stress pathway.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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