The effects of low-level laser light exposure on sperm motion characteristics and DNA damage
Firestone RS, Esfandiari N, Moskovtsev SI, Burstein E, Videna GT, Librach C, Bentov Y, Casper RF
Journal of andrology · 38 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Health markers and function
Who paid for it
- Funding
- Funding not disclosed
Publication
- Published
- 2012-05-06 · J Androl · vol. 33 · issue 3 · pp. 469–473
- Cited
- 63 citations · more than 85% of similar papers · 1.8× the field average
- References
- 23 works
- Access
- Paywalled
- Research areas
- Sperm and Testicular Function · Laser Applications in Dentistry and Medicine · Bee Products Chemical Analysis
- Keywords
- Semen, Sperm, DNA fragmentation, Andrology, Sperm motility, Semen analysis, Motility, DNA damage, Chemistry, Biology, Molecular biology, DNA, Apoptosis, Medicine, Biochemistry, Genetics, Programmed cell death
- MeSH
- humans, oligospermia, dna damage, cryopreservation, lasers, infrared rays, dna fragmentation, sperm motility, male, asthenozoospermia
8 authors
From CA
- Ross FirestoneMount Sinai Hospital; Lunenfeld-Tanenbaum Research Institute
- Navid EsfandiariMount Sinai Hospital; University of Toronto; Lunenfeld-Tanenbaum Research Institute
- Sergey I. MoskovtsevUniversity of Toronto; CReATe Fertility Centre
- Eliezer BursteinMount Sinai Hospital; Lunenfeld-Tanenbaum Research Institute
- German T. VidenaCReATe Fertility Centre
- Clifford Lawrence LibrachUniversity of Toronto; CReATe Fertility Centre
Abstract
The objective of this study was to determine the effects of low-level laser light exposure on the motility of spermatozoa and on DNA damage. Thirty-three semen samples were collected for routine analysis and were classified as normospermic, oligospermic, or asthenospermic. After routine semen analysis was performed, residual semen was divided into treated and control aliquots. Treated samples were exposed to a 30-second infrared laser pulse of 50 mW/cm(2) at 905 nm, a wavelength thought to increase light-sensitive cytochrome c oxidase in the mitochondrial electron transport chain. Samples were then incubated at 37°C, and aliquots were analyzed at 30 minutes and 2 hours using computerassisted semen analysis. After incubation, 250 μL of each sample was frozen at 280°C until DNA fragmentation analysis by flow cytometry. A significant increase in motility, most prominent in oligospermic and asthenospermic samples (85% increase), was observed 30 minutes after the treatment (P < .0001). No significant increase in DNA damage compared with control samples was observed. Significant changes in sperm motion kinetics were observed. Low-level laser light exposure appears to have a positive short-term effect on the motility of treated spermatozoa and did not cause any increase in DNA damage measured at 2 hours. We conclude that some cases of asthenospermia may be related to mitochondrial dysfunction. The implications of this study in terms of future clinical applications needs further investigation.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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