Methylation patterns in sentinel genes in peripheral blood cells of heavy smokers: Influence of cruciferous vegetables in an intervention study
Scoccianti C, Ricceri F, Ferrari P, Cuenin C, Sacerdote C, Polidoro S, Jenab M, Hainaut P, Vineis P, Herceg Z
Epigenetics · 29 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Randomized controlled trial (indexed by PubMed)
- Studied in
- People
- Main outcome
- Mechanisms only
- Intake measured by
- Not stated
Who paid for it
- Funding
- Independent funding
- Nonprofit
- World Cancer Research Fund
- Government
- Medical Research Council
Based on 2 listed funder(s).
Publication
- Published
- 2011-09-01 · Epigenetics · vol. 6 · issue 9 · pp. 1114–1119
- Publisher
- Landes Bioscience
- Cited
- 41 citations · more than 82% of similar papers · 1.6× the field average
- References
- 19 works
- Access
- Free to read
- Research areas
- Epigenetics and DNA Methylation · Family Support in Illness
- Keywords
- Biology, DNA methylation, Methylation, Cruciferous vegetables, Epigenetics, Methylenetetrahydrofolate reductase, CDKN2A, Bisulfite sequencing, Carcinogenesis, Physiology, Genetics, Gene, Internal medicine, Cancer, Allele, Gene expression, Medicine
- MeSH
- leukocytes, humans, brassicaceae, isothiocyanates, flavonoids, methylenetetrahydrofolate reductase (nadph2), adaptor proteins, signal transducing, tumor suppressor proteins, nuclear proteins, diet, sequence analysis, dna, smoking, dna methylation, epigenesis, genetic, long interspersed nucleotide elements, genes, p16, middle aged, male, mutl protein homolog 1, glycine max
10 authors
From FR, IT, GB
- Chiara ScocciantiCentre international de recherche sur le cancer
- Fulvio RicceriItalian institute for Genomic Medicine
- Pietro FerrariCentre international de recherche sur le cancer
- Cyrille CueninCentre international de recherche sur le cancer
- Carlotta SacerdoteItalian institute for Genomic Medicine; Piedmont Reference Center for Epidemiology and Cancer Prevention
- Silvia PolidoroItalian institute for Genomic Medicine
Abstract
Changes in DNA methylation patterns are a hallmark of tobacco-induced carcinogenesis. We have conducted a randomized 4-week intervention trial to investigate the effects of three dietary regimens to modify DNA methylation patterns in peripheral white blood cells of heavy smokers. A group of 88 smokers were randomly assigned to and distributed among three diets, including (1) normal isocaloric diet (balanced in fruits and vegetables), according to international guidelines; (2) a diet enriched in flavonoids and isothiocyanates (particularly cruciferous vegetables); (3) a regimen consisting of diet 1 supplemented with flavonoids (green tea and soy products). Methylation patterns were analyzed by pyrosequencing in LINE1 (Long Interspersed DNA Elements), RASSF1A, ARF and CDKN2a (tumor suppressor genes), MLH1 (mismatch DNA repair) and MTHFR (folate metabolism). Three distinct patterns of methylation were observed. In LINE1, methylation showed a small but reproducible increase with all three regimens. MTHFR was constitutively methylated with no significant modulation by diets. The four other loci showed low basal levels of methylation with no substantial change after intervention. These data suggest that the isocaloric diet may stabilize global epigenetic (LINE1 DNA methylation) patterns in peripheral white blood cells but does not provide evidence for methylation changes in specific genes associated with this short-term dietary intervention.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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