Comparison of isothiocyanate metabolite levels and histone deacetylase activity in human subjects consuming broccoli sprouts or broccoli supplement
Clarke JD, Riedl K, Bella D, Schwartz SJ, Stevens JF, Ho E
Journal of agricultural and food chemistry · 52 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Controlled clinical trial (classified by our AI screen)
- Studied in
- People
- Main outcome
- Mechanisms only
- Intake measured by
- Food provided by researchers
Who paid for it
- Funding
- Independent funding
- Government
- National Cancer Institute
- Government
- National Institute of Environmental Health Sciences
- Government
- NIEHS NIH HHS
- Government
- NCI NIH HHS
- Grants
- National Cancer Institute (R01 CA-122906); National Cancer Institute (P30CA016058); National Cancer Institute (CA 090890); National Institute of Environmental Health Sciences (P30ES000210); National Institute of Environmental Health Sciences (#P30-ES00210); National Cancer Institute (P01 CA090890); National Cancer Institute (CA122906)
Based on 4 listed funder(s).
Publication
- Published
- 2011-09-30 · J Agric Food Chem · vol. 59 · issue 20 · pp. 10955–10963
- Publisher
- American Chemical Society
- Cited
- 78 citations · more than 90% of similar papers · 2.6× the field average
- Impact
- Top 10% most cited in its field
- References
- 33 works
- Access
- Open access (repository copy)
- Research areas
- Genomics, phytochemicals, and oxidative stress · Synthesis and Biological Evaluation
- Keywords
- Sulforaphane, Myrosinase, Glucosinolate, Cruciferous vegetables, Isothiocyanate, Glucoraphanin, Chemistry, Food science, Mercapturic acid, Excretion, Metabolite, Allyl isothiocyanate, Brassica, Biochemistry, Botany, Glutathione, Biology, Internal medicine, Medicine, Enzyme
- MeSH
- humans, brassica, plant shoots, sulfides, oximes, thiocyanates, imidoesters, isothiocyanates, sulfoxides, histone deacetylases, glucosinolates, glucose, diet, dietary supplements, adult, middle aged, female, male
6 authors
From US
- John ClarkeOregon State University
- Kenneth M. RiedlThe Ohio State University
- Deborah L. BellaOregon State University; The Ohio State University
- Steven J. SchwartzThe Ohio State University
- Jan Frederik StevensOregon State University; The Ohio State University
- Emily H. Ho · correspondingOregon State University; The Ohio State University
Abstract
Increased consumption of cruciferous vegetables such as broccoli may reduce the risk of various cancers. Myrosinase is required to convert dietary glucosinolates from broccoli into bioactive isothiocyanates. We evaluated isothiocyanate excretion profiles in healthy subjects who consumed broccoli sprouts or broccoli supplement (no myrosinase) with equivalent glucosinolate content. Urinary metabolites of two major isothiocyanates, sulforaphane and erucin, were measured by liquid chromatography coupled with tandem mass spectrometry. Peak excretion of sulforaphane and erucin was higher and occurred sooner in subjects who consumed broccoli sprouts as compared to subjects who consumed the supplement. A subject-dependent shift in the ratio of urinary sulforaphane to erucin metabolites was observed in both groups, indicating conversion of sulforaphane to erucin. Lower histone deacetylase activity was observed in the peripheral blood mononuclear cells only in subjects consuming sprouts. Fresh broccoli sprouts differ from broccoli supplements in regards to excretion of isothiocyanates and bioactivity in human subjects.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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