Modulation of mouse gastrointestinal motility by allyl isothiocyanate, a constituent of cruciferous vegetables (Brassicaceae): evidence for TRPA1-independent effects
Capasso R, Aviello G, Romano B, Borrelli F, De Petrocellis L, Di Marzo V, Izzo AA
British journal of pharmacology · 38 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Animal study (classified by our AI screen)
- Studied in
- Animals
- Main outcome
- Health markers and function
Who paid for it
- Funding
- Funding not disclosed
Publication
- Published
- 2011-09-28 · Br J Pharmacol · vol. 165 · issue 6 · pp. 1966–1977
- Publisher
- Wiley
- Cited
- 51 citations · more than 87% of similar papers · 2.5× the field average
- References
- 49 works
- Access
- Free to read
- Research areas
- Ion Channels and Receptors · Phytochemicals and Antioxidant Activities · Postharvest Quality and Shelf Life Management
- Keywords
- Allyl isothiocyanate, Chemistry, Motility, Pharmacology, Contractility, Ryanodine receptor, In vivo, Thapsigargin, Biochemistry, Biology, Intracellular, Endocrinology, Cell biology
- MeSH
- colon, ileum, animals, mice, inbred icr, humans, mice, brassicaceae, vegetables, isothiocyanates, parasympatholytics, gastrointestinal motility, muscle contraction, male, transient receptor potential channels, hek293 cells, in vitro techniques, trpa1 cation channel
7 authors
From IT
- Raffaele CapassoUniversity of Naples Federico II
- Gabriella AvielloUniversity of Naples Federico II
- Barbara RomanoUniversity of Naples Federico II
- Francesca BorrelliUniversity of Naples Federico II
- Luciano De PetrocellisNational Research Council
- Vincenzo Di Marzo · correspondingNational Research Council
Abstract
Background and purpose
Allyl isothiocyanate (AITC, mustard oil), a constituent of many common cruciferous vegetables (Brassicaceae), activates transient receptor potential of ankyrin type-1 (TRPA1) channels, claimed to regulate gastrointestinal contractility. In this study, we have investigated the effect of AITC on intestinal motility.
Experimental approach
Effects of AITC were investigated in vivo on upper gastrointestinal transit in mice and in mouse isolated ileum [contractions induced by electrical field stimulation (EFS), acetylcholine and spontaneous contractility]. The contractor activity of AITC was studied in mouse isolated colon. The ability of TRPA1 channel antagonists to block AITC-induced elevation of intracellular Ca(2+) [Ca(2+)](i) was assessed in HEK293 cells transfected with rat TRPA1 channels.
Key results
AITC increased [Ca(2+)](i) in HEK293 cells, reduced ileal contractility (acetylcholine-, EFS-induced contractions and spontaneous contractility), but contracted the isolated colon. Gentamicin and camphor (non-selective TRPA1 channel antagonists), HC-030031 and AP18 (selective TRPA1 channel agonists) inhibited AITC-induced effects in HEK293 cells but not in the ileum or colon. AITC-induced contractions were reduced by tetrodotoxin and strongly reduced by nifedipine, cyclopiazonic acid and ryanodine. In vivo, AITC reduced (following i.p. administration) or increased (following intragastric administration) upper gastrointestinal transit in mice These effects were not affected by HC-030031.
Conclusion and implications
AITC, depending, in vitro, on the regions of gut examined and, in vivo, on the route of administration, exerted both stimulatory and inhibitory effects on intestinal motility, which were not sensitive to TRPA1 channel antagonists. The proposition that TRPA1 channels are the primary targets for AITC to induce contraction should be revised.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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