Effect of blueberry ingestion on natural killer cell counts, oxidative stress, and inflammation prior to and after 2.5 h of running
McAnulty LS, Nieman DC, Dumke CL, Shooter LA, Henson DA, Utter AC, Milne G, McAnulty SR
Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme · 93 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Randomized controlled trial (indexed by PubMed)
- Studied in
- People
- Main outcome
- Mechanisms only
- Intake measured by
- Food provided by researchers
Who paid for it
- Funding
- Funding not disclosed
Publication
- Published
- 2011-12-01 · Appl Physiol Nutr Metab · vol. 36 · issue 6 · pp. 976–984
- Publisher
- NRC Research Press
- Cited
- 142 citations · more than 96% of similar papers · 5.9× the field average
- Impact
- Top 10% most cited in its field
- References
- 38 works
- Access
- Paywalled
- Research areas
- Exercise and Physiological Responses · Antioxidant Activity and Oxidative Stress · Vitamin C and Antioxidants Research
- Keywords
- Oxidative stress, Inflammation, Urine, Antioxidant, Immune system, Endocrinology, Ingestion, Internal medicine, Ferric reducing ability of plasma, Natural killer cell, Medicine, Chemistry, Immunology, Biochemistry, Antioxidant capacity, Cytotoxic T cell
- MeSH
- killer cells, natural, humans, fruit, myositis, f2-isoprostanes, thymidine, anti-inflammatory agents, non-steroidal, interleukin-10, antioxidants, lymphocyte count, exercise, oxidative stress, physical endurance, running, adolescent, adult, young adult, athletes, biomarkers, blueberry plants
8 authors
From US
- Lisa S. McAnultyAppalachian State University
- David Christopher NiemanAppalachian State University
- Charles L. DumkeUniversity of Montana
- Lesli ShooterAppalachian State University
- Dru Anne HensonAppalachian State University
- Alan C. UtterAppalachian State University
Abstract
Blueberries are rich in antioxidants known as anthocyanins, which may exhibit significant health benefits. Strenous exercise is known to acutely generate oxidative stress and an inflammatory state, and serves as an on-demand model to test antioxidant and anti-inflammatory compounds. The purpose of this study was to examine whether 250 g of blueberries per day for 6 weeks and 375 g given 1 h prior to 2.5 h of running at ∼72% maximal oxygen consumption counters oxidative stress, inflammation, and immune changes. Twenty-five well-trained subjects were recruited and randomized into blueberry (BB) (N = 13) or control (CON) (N = 12) groups. Blood, muscle, and urine samples were obtained pre-exercise and immediately postexercise, and blood and urine 1 h postexercise. Blood was examined for F₂-isoprostanes for oxidative stress, cortisol, cytokines, homocysteine, leukocytes, T-cell function, natural killer (NK), and lymphocyte cell counts for inflammation and immune system activation, and ferric reducing ability of plasma for antioxidant capacity. Muscle biopsies were examined for glycogen and NFkB expression to evaluate stress and inflammation. Urine was tested for modification of DNA (8-OHDG) and RNA (5-OHMU) as markers of nucleic acid oxidation. A 2 (treatment) × 3 (time) repeated measures ANOVA was used for statistical analysis. Increases in F₂-isoprostanes and 5-OHMU were significantly less in BB and plasma IL-10 and NK cell counts were significantly greater in BB vs. CON. Changes in all other markers did not differ. This study indicates that daily blueberry consumption for 6 weeks increases NK cell counts, and acute ingestion reduces oxidative stress and increases anti-inflammatory cytokines.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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