Effect of low-level laser irradiation on osteoblast proliferation and bone formation
Grassi FR, Ciccolella F, D'Apolito G, Papa F, Iuso A, Salzo AE, Trentadue R, Nardi GM, Scivetti M, De Matteo M, Silvestris F, Ballini A, Inchingolo F, Dipalma G, Scacco S, Tetè S
Journal of biological regulators and homeostatic agents · 35 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Funding not disclosed
Publication
- Published
- 2012-02-09 · J Biol Regul Homeost Agents · vol. 25 · issue 4 · pp. 603–14
- Publisher
- National Institutes of Health
- Cited
- 51 citations · more than 91% of similar papers · 3.0× the field average
- Impact
- Top 10% most cited in its field
- References
- 0 works
- Access
- Paywalled
- Research areas
- Laser Applications in Dentistry and Medicine
- Keywords
- Osteoblast, Alkaline phosphatase, RUNX2, Cell growth, Irradiation, Chemistry, Cancer research, Andrology, Cell biology, Molecular biology, Medicine, Biology, Biochemistry, Enzyme, In vitro
- MeSH
- bone matrix, cells, cultured, osteoblasts, humans, transcription factors, cell proliferation, cell respiration, osteogenesis, adult, middle aged, core binding factor alpha 1 subunit, low-level light therapy, sp7 transcription factor
17 authors
From IT
- Felice Roberto GrassiUniversity of Bari Aldo Moro
- F Ciccolella
- G D'Apolito
- F Papa
- Arcangela Iuso
- A E Salzo
Abstract
Applications of laser therapy in biostimulation and healing injured tissues are widely described in medical literature. The present study focuses on the effects of laser irradiation on the growth rate and differentiation of human osteoblast-like cells seeded on titanium or zirconia surfaces. Cells were laser irradiated with low therapeutical doses at different intervals and the effects of irradiation were evaluated at each time-point. After 3 hours lasered cells showed an enhanced mitogen activity compared to non-lasered control cells and a higher alkaline phosphatase activity, marker of bone formation. At the same time, the mRNA of RUNX2 and OSTERIX, two genes involved in osteoblast differentiation, showed a clear decrease in lasered cells. This reached the lowest value 6 to 12 hours after irradiation, after which the transcripts started to increase, indicating that the laser treatment did promote the osteogenic potential of growth-induced cells. These results indicate that Low Level Laser Treatment (LLLT) stimulates osteogenic cell proliferation.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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