Water extract from the leaves of Withania somnifera protect RA differentiated C6 and IMR-32 cells against glutamate-induced excitotoxicity
Kataria H, Wadhwa R, Kaul SC, Kaur G
PloS one · 46 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- National Institute of Advanced Industrial Science and Technology
- Government
- Department of Biotechnology, Ministry of Science and Technology, India
- Government
- Council of Scientific and Industrial Research, India
Based on 3 listed funder(s).
Publication
- Published
- 2012-05-14 · PLoS One · vol. 7 · issue 5 · p. e37080
- Publisher
- Public Library of Science
- Cited
- 77 citations · more than 99% of similar papers · 19.8× the field average
- Impact
- Top 10% most cited in its field
- References
- 86 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Phytochemicals and Medicinal Plants · Medicinal Plants and Neuroprotection · Neuroscience and Neural Engineering
- Keywords
- Glutamate receptor, Withania somnifera, Neuroprotection, Neurotoxicity, Excitotoxicity, Programmed cell death, Viability assay, Biology, Neurodegeneration, Cell biology, Neural cell adhesion molecule, Pharmacology, Biochemistry, Chemistry, Medicine, Cell, Apoptosis, Cell adhesion, Toxicity, Internal medicine, Pathology
- MeSH
- neurons, cell line, animals, humans, rats, withania, plant leaves, plants, medicinal, nerve degeneration, tretinoin, glial fibrillary acidic protein, neurofilament proteins, glutamic acid, neural cell adhesion molecules, excitatory amino acid antagonists, neuroprotective agents, plant extracts, neurotoxins, cell death, cell differentiation, hsp70 heat-shock proteins, matrix metalloproteinase 2, matrix metalloproteinase 9
4 authors
From IN, JP
- Hardeep KatariaGuru Nanak Dev University
- Renu Wadhwa · correspondingNational Institute of Advanced Industrial Science and Technology
- Sunil C. KaulNational Institute of Advanced Industrial Science and Technology
- Gurcharan Kaur · correspondingGuru Nanak Dev University
Abstract
Glutamate neurotoxicity has been implicated in stroke, head trauma, multiple sclerosis and neurodegenerative disorders. Search for herbal remedies that may possibly act as therapeutic agents is an active area of research to combat these diseases. The present study was designed to investigate the neuroprotective role of Withania somnifera (Ashwagandha), also known as Indian ginseng, against glutamate induced toxicity in the retinoic acid differentiated rat glioma (C6) and human neuroblastoma (IMR-32) cells. The neuroprotective activity of the Ashwagandha leaves derived water extract (ASH-WEX) was evaluated. Cell viability and the expression of glial and neuronal cell differentiation markers was examined in glutamate challenged differentiated cells with and without the presence of ASH-WEX. We demonstrate that RA-differentiated C6 and IMR-32 cells, when exposed to glutamate, undergo loss of neural network and cell death that was accompanied by increase in the stress protein HSP70. ASH-WEX pre-treatment inhibited glutamate-induced cell death and was able to revert glutamate-induced changes in HSP70 to a large extent. Furthermore, the analysis on the neuronal plasticity marker NCAM (Neural cell adhesion molecule) and its polysialylated form, PSA-NCAM revealed that ASH-WEX has therapeutic potential for prevention of neurodegeneration associated with glutamate-induced excitotoxicty.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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