Antiproliferative activity of the dietary isothiocyanate erucin, a bioactive compound from cruciferous vegetables, on human prostate cancer cells
Melchini A, Traka MH, Catania S, Miceli N, Taviano MF, Maimone P, Francisco M, Mithen RF, Costa C
Nutrition and cancer · 27 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Funding not disclosed
Publication
- Published
- 2013-01-01 · Nutr Cancer · vol. 65 · issue 1 · pp. 132–138
- Publisher
- Routledge
- Cited
- 42 citations · more than 91% of similar papers · 2.7× the field average
- Impact
- Top 10% most cited in its field
- References
- 38 works
- Access
- Paywalled
- Research areas
- Genomics, phytochemicals, and oxidative stress · Kruppel-like factors research · Glutathione Transferases and Polymorphisms
- Keywords
- Cruciferous vegetables, Protein kinase B, Sulforaphane, MAPK/ERK pathway, Cell growth, Cancer research, Kinase, Cyclin-dependent kinase, Prostate cancer, Isothiocyanate, Signal transduction, Cancer, Carcinogenesis, PI3K/AKT/mTOR pathway, Phenethyl isothiocyanate, Chemistry, Pharmacology, Biology, Biochemistry, Medicine, Internal medicine, Cell cycle
- MeSH
- cell line, tumor, humans, vegetables, adenocarcinoma, prostatic neoplasms, sulfides, thiocyanates, isothiocyanates, sulfoxides, extracellular signal-regulated map kinases, anticarcinogenic agents, antineoplastic agents, phytogenic, cell proliferation, dose-response relationship, drug, male, cyclin-dependent kinase inhibitor p21, proto-oncogene proteins c-akt, phosphatidylinositol 3-kinases
9 authors
From IT, GB, ES
- Antonietta MelchiniUniversity of Messina; Norwich Research Park; Quadram Institute
- Μαρία ΤράκαNorwich Research Park; Quadram Institute
- Stefania CataniaUniversity of Messina
- Natalizia MiceliUniversity of Messina
- Maria Fernanda TavianoUniversity of Messina
- Patrizia MaimoneUniversity of Messina
Abstract
It is becoming increasingly clear that many dietary agents, such as isothiocyanates (ITCs) from cruciferous vegetables, can retard or prevent the process of prostate carcinogenesis. Erucin (ER) is a dietary ITC, which has been recently considered a promising cancer chemopreventive phytochemical. The potential protective activity of ER against prostate cancer was investigated using prostate adenocarcinoma cells (PC3), to analyze its effects on pathways involved in cell growth regulation, such as the cyclin-dependent kinase (CDKs) inhibitor p21(WAF1/CIP1) (p21), phosphatidylinositol-3 kinase/AKT, and extracellular signal-regulated kinases (ERK)1/2 signaling pathways. We have shown for the first time that ER increases significantly p21 protein expression and ERK1/2 phosphorylation in a dose-dependent manner to inhibit PC3 cell proliferation (P ≤ 0.01). Compared to the structurally related sulforaphane, a well-studied broccoli-derived ITC, ER showed lower potency in inhibiting proliferation of PC3 cells, as well as in modulating p21 and pERK1/2 protein levels. Neither of the naturally occurring ITCs was able to affect significantly pAKT protein levels in prostate cells at all concentrations tested (0-25 μM). It is clearly important for the translation of laboratory findings to clinical approaches to investigate in animal and cell studies the molecular mechanisms by which ITCs may exert health promoting effects.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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