Withania somnifera water extract as a potential candidate for differentiation based therapy of human neuroblastomas
Kataria H, Wadhwa R, Kaul SC, Kaur G
PloS one · 25 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- National Institute of Advanced Industrial Science and Technology
- Government
- Department of Biotechnology, Ministry of Science and Technology, India
- Government
- Council of Scientific and Industrial Research, India
Based on 3 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2013-01-31 · PLoS One · vol. 8 · issue 1 · p. e55316
- Publisher
- Public Library of Science
- Cited
- 45 citations · more than 98% of similar papers · 14.6× the field average
- Impact
- Top 10% most cited in its field
- References
- 82 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Phytochemicals and Medicinal Plants · Cell death mechanisms and regulation · Neuroblastoma Research and Treatments
- Keywords
- Withania somnifera, Neuroblastoma, Biology, Apoptosis, Cell cycle, Cancer research, Cell growth, Cellular differentiation, Cell biology, Medicine, Cell culture, Pathology, Biochemistry
- MeSH
- cell line, tumor, humans, withania, neuroblastoma, water, fluorescein-5-isothiocyanate, annexin a5, neural cell adhesion molecules, dna primers, plant extracts, blotting, western, phytotherapy, reverse transcriptase polymerase chain reaction, cell differentiation, cell proliferation, hsp70 heat-shock proteins, cell cycle checkpoints
4 authors
From IN, JP
- Hardeep KatariaGuru Nanak Dev University
- Renu WadhwaNational Institute of Advanced Industrial Science and Technology
- Sunil C. KaulNational Institute of Advanced Industrial Science and Technology
- Gurcharan Kaur · correspondingGuru Nanak Dev University
Abstract
Neuroblastoma is an aggressive childhood disease of the sympathetic nervous system. Treatments are often ineffective and have serious side effects. Conventional therapy of neuroblastoma includes the differentiation agents. Unlike chemo-radiotherapy, differentiation therapy shows minimal side effects on normal cells, because normal non-malignant cells are already differentiated. Keeping in view the limited toxicity of Withania somnifera (Ashwagandha), the current study was aimed to investigate the efficacy of Ashwagandha water extract (ASH-WEX) for anti-proliferative potential in neuroblastoma and its underlying signalling mechanisms. ASH-WEX significantly reduced cell proliferation and induced cell differentiation as indicated by morphological changes and NF200 expression in human IMR-32 neuroblastoma cells. The induction of differentiation was accompanied by HSP70 and mortalin induction as well as pancytoplasmic translocation of the mortalin in ASH-WEX treated cells. Furthermore, the ASH-WEX treatment lead to induction of neural cell adhesion molecule (NCAM) expression and reduction in its polysialylation, thus elucidating its anti-migratory potential, which was also supported by downregulation of MMP 2 and 9 activity. ASH-WEX treatment led to cell cycle arrest at G0/G1 phase and increase in early apoptotic population. Modulation of cell cycle marker Cyclin D1, anti-apoptotic marker bcl-xl and Akt-P provide evidence that ASH-WEX may prove to be a promising phytotherapeutic intervention in neuroblatoma related malignancies.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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