Phototherapy with low-level laser influences the proliferation of endothelial cells and vascular endothelial growth factor and transforming growth factor-beta secretion
Szymanska J, Goralczyk K, Klawe JJ, Lukowicz M, Michalska M, Goralczyk B, Zalewski P, Newton JL, Gryko L, Zajac A, Rosc D
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society · 57 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Funding not disclosed
Publication
- Published
- 2013-06-01 · J Physiol Pharmacol · vol. 64 · issue 3 · pp. 387–91
- Publisher
- National Institutes of Health
- Cited
- 80 citations · more than 97% of similar papers · 6.8× the field average
- Impact
- Top 10% most cited in its field
- References
- 30 works
- Access
- Paywalled
- Research areas
- Laser Applications in Dentistry and Medicine · Medical and Biological Ozone Research
- Keywords
- Angiogenesis, Vascular endothelial growth factor, Endothelial stem cell, Cell growth, Secretion, Transforming growth factor, Vascular endothelial growth factor A, Chemistry, Growth factor, Transforming growth factor beta, Stimulation, Endocrinology, Biophysics, Internal medicine, Biology, In vitro, Medicine, VEGF receptors, Biochemistry
- MeSH
- endothelium, vascular, cells, cultured, humans, vascular endothelial growth factor a, neoplasm proteins, cell count, infrared rays, dose-response relationship, radiation, wound healing, cell proliferation, down-regulation, up-regulation, neovascularization, physiologic, light, transforming growth factor beta1, secretory pathway, human umbilical vein endothelial cells, low-level light therapy
11 authors
From PL
- Justyna SzymańskaNicolaus Copernicus University
- Krzysztof Góralczyk
- J J Klawe
- Małgorzata Łukowicz
- Małgorzata Michalska
- Barbara Góralczyk
Abstract
The healing process and the angiogenesis associated with it, is a very important but currently poorly understood area. Low level laser therapy (LLLT) has been reported to modulate the process of tissue repair by stimulation of cellular reaction such as migration, proliferation, apoptosis and cellular differentiation. The aim of this work was to evaluate the influence of laser radiation in the range of visible and infrared light on the proliferation of vascular endothelial cells in vitro and the secretion of angiogenic factors: vascular endothelial growth factor (VEGF)-A and transforming growth factor (TGF)-β. Vascular human endothelial cells (Ecs) were exposed to radiation with laser beam of the wavelengths: 635 nm (1.875 mW/cm²) and 830 nm (3.75 mW/cm²). Depending on the radiation energy density, the experiment was conducted in four groups : I) the control group (no radiation, 0 J/cm²); II) 635 nm - the energy density was 2 J/cm²; III) 635 nm - 4 J/cm²; IV635 nm - 8 J/cm², II) 830 nm - the energy density was 2 J/cm²; III) 830 nm - 4 J/cm²; IV) 830 nm - 8 J/cm². The proliferation and concentration of VEGF-A and TGF-β were examined. LLLT with wavelength 635 nm increases endothelial cell proliferation. Significant increase in endothelial cell proliferation and corresponding decrease in VEGF concentration may suggest the role for VEGF in this process. The wavelength of 830 nm was associated with a decrease in TGF-β secretion.
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