A diet rich in high-glucoraphanin broccoli interacts with genotype to reduce discordance in plasma metabolite profiles by modulating mitochondrial function
Armah CN, Traka MH, Dainty JR, Defernez M, Janssens A, Leung W, Doleman JF, Potter JF, Mithen RF
The American journal of clinical nutrition · 53 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Randomized controlled trial (indexed by PubMed)
- Studied in
- People
- Main outcome
- Health markers and function
- Intake measured by
- Food provided by researchers
Who paid for it
- Funding
- Independent funding
- Nonprofit
- Prostate Cancer Foundation
- Government
- Directorate for Biological Sciences
- Government
- Biotechnology and Biological Sciences Research Council
- Authors
- At least one author declares a financial tie to industry
- Grants
- Biotechnology and Biological Sciences Research Council (BBS/E/F/00044431); Biotechnology and Biological Sciences Research Council (BBS/E/F/00044431); Biotechnology and Biological Sciences Research Council (BB/J004545/1)
Based on 3 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2013-08-20 · Am J Clin Nutr · vol. 98 · issue 3 · pp. 712–722
- Publisher
- Elsevier BV
- Cited
- 77 citations · more than 94% of similar papers · 3.9× the field average
- Impact
- Top 10% most cited in its field
- References
- 57 works
- Access
- Open access (hybrid journal) · CC-BY
- Research areas
- Genomics, phytochemicals, and oxidative stress · Liver Disease Diagnosis and Treatment · Alcohol Consumption and Health Effects
- Keywords
- Glucoraphanin, Metabolite, Metabolome, Sulforaphane, Genotype, Biology, Metabolomics, Cruciferous vegetables, Internal medicine, Endocrinology, Food science, Chemistry, Biochemistry, Medicine, Bioinformatics, Brassica, Glucosinolate, Botany, Cancer, Gene
- MeSH
- mitochondria, humans, brassica, cardiovascular diseases, oximes, imidoesters, sulfoxides, nucleotidyltransferases, glucosinolates, lipids, amino acids, plant extracts, diet, multivariate analysis, sex factors, genotype, polymorphism, single nucleotide, aged, middle aged, female, male, metabolome, biomarkers
9 authors
From GB
- Charlotte N. ArmahNorwich Research Park; Quadram Institute
- Μαρία ΤράκαUniversity of East Anglia; Norwich Research Park
- Jack R. DaintyUniversity of East Anglia; Norwich Research Park
- Marianne DefernezUniversity of East Anglia; Norwich Research Park
- Astrid JanssensUniversity of East Anglia; Norwich Research Park
- W. LeungUniversity of East Anglia
Abstract
Background
Observational and experimental studies suggest that diets rich in cruciferous vegetables and glucosinolates may reduce the risk of cancer and cardiovascular disease (CVD).
Objective
We tested the hypothesis that a 12-wk dietary intervention with high-glucoraphanin (HG) broccoli would modify biomarkers of CVD risk and plasma metabolite profiles to a greater extent than interventions with standard broccoli or peas.
Design
Subjects were randomly assigned to consume 400 g standard broccoli, 400 g HG broccoli, or 400 g peas each week for 12 wk, with no other dietary restrictions. Biomarkers of CVD risk and 347 plasma metabolites were quantified before and after the intervention.
Results
No significant differences in the effects of the diets on biomarkers of CVD risk were found. Multivariate analyses of plasma metabolites identified 2 discrete phenotypic responses to diet in individuals within the HG broccoli arm, differentiated by single nucleotide polymorphisms associated with the PAPOLG gene. Univariate analysis showed effects of sex (P < 0.001), PAPOLG genotype (P < 0.001), and PAPOLG genotype × diet (P < 0.001) on the plasma metabolic profile. In the HG broccoli arm, the consequence of the intervention was to reduce variation in lipid and amino acid metabolites, tricarboxylic acid (TCA) cycle intermediates, and acylcarnitines between the 2 PAPOLG genotypes.
Conclusions
The metabolic changes observed with the HG broccoli diet are consistent with a rebalancing of anaplerotic and cataplerotic reactions and enhanced integration of fatty acid β-oxidation with TCA cycle activity. These modifications may contribute to the reduction in cancer risk associated with diets that are rich in cruciferous vegetables. This trial was registered at clinicaltrials.gov as NCT01114399.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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