Effect of 940 nm low-level laser therapy on osteogenesis in vitro
Jawad MM, Husein A, Azlina A, Alam MK, Hassan R, Shaari R
Journal of biomedical optics · 48 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- University or hospital
- Universiti Sains Malaysia
Based on 1 listed funder(s).
Publication
- Published
- 2013-12-10 · J Biomed Opt · vol. 18 · issue 12 · p. 128001
- Publisher
- SPIE
- Cited
- 66 citations · more than 90% of similar papers · 2.9× the field average
- References
- 51 works
- Access
- Free to read
- Research areas
- Laser Applications in Dentistry and Medicine · Dental Implant Techniques and Outcomes · Bone Tissue Engineering Materials
- Keywords
- Alkaline phosphatase, Osteocalcin, Low level laser therapy, Osteoblast, Cell growth, Chemistry, Bone healing, MTT assay, Cell, Cellular differentiation, In vitro, Dentistry, Laser therapy, Andrology, Medicine, Surgery, Laser, Biochemistry, Enzyme
- MeSH
- cell line, osteoblasts, humans, alkaline phosphatase, osteocalcin, cell differentiation, cell proliferation, osteogenesis, lasers, semiconductor, low-level light therapy
6 authors
From MY
- Mohammed Mahmood JawadUniversiti Sains Malaysia
- Adam HuseinUniversiti Sains Malaysia
- Ahmad AzlinaHospital Universiti Sains Malaysia
- Mohammad Khursheed AlamHospital Universiti Sains Malaysia
- Rozita HassanHospital Universiti Sains Malaysia
- Rumaizi ShaariUniversiti Malaysia Kelantan
Abstract
Bone regeneration is essential in medical treatment, such as in surgical bone healing and orthodontics. The aim of this study is to examine the effect of different powers of 940 nm diode low-level laser treatment (LLLT) on osteoblast cells during their proliferation and differentiation stages. A human fetal osteoblast cell line was cultured and treated with LLLT. The cells were divided into experimental groups according to the power delivered and periods of exposure per day for each laser power. The (3-(4,5-dimethylthiazol-2yl)-2,5 diphenyl tetrazolium bromide) (MTT) assay was used to determine cell proliferation. Both alkaline phosphatase and osteocalcin activity assays were assessed for cell differentiation. All treatment groups showed a significant increase in cell proliferation and differentiation compared to the control group. Regarding the exposure time, the subgroups treated with the LLLT for 6 min showed higher proliferation and differentiation rates for the powers delivered, the 300-mW LLLT group significantly increased the amount of cell proliferation. By contrast, the 100 and 200 mW groups showed significantly greater amounts of cell differentiation. These results suggest that the use of LLLT may play an important role in stimulating osteoblast cells for improved bone formation.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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