Effects of low-level laser therapy on M1-related cytokine expression in monocytes via histone modification
Chen CH, Wang CZ, Wang YH, Liao WT, Chen YJ, Kuo CH, Kuo HF, Hung CH
Mediators of inflammation · 35 citations
Review labels
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How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- University or hospital
- Kaohsiung Municipal Ta-Tung Hospital
- Government
- National Institute for Health and Care Research
- Government
- National Science Council
- Government
- National Health Research Institutes
- Government
- National Institute for Health Research (NIHR)
- Grants
- National Institute for Health and Care Research (EX102-9914EC); National Health Research Institutes (NSC 101-); Kaohsiung Municipal Ta-Tung Hospital (NSC 101-2314-B-037 -002); National Science Council (KMTTH-101-012); Kaohsiung Municipal Ta-Tung Hospital (KMTTH-101-012); National Health Research Institutes (NHRI-EX102); National Science Council (EX102-9914EC); National Science Council (NSC 101-2314-B-037 -002); Kaohsiung Municipal Ta-Tung Hospital (EX102-9914EC); National Institute for Health and Care Research (NSC 101-2314-B-037 -002); National Institute for Health and Care Research (KMTTH-101-012)
Based on 5 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2014-01-01 · Mediators Inflamm · vol. 2014 · pp. 1–13
- Publisher
- Hindawi Publishing Corporation
- Cited
- 62 citations · more than 94% of similar papers · 4.1× the field average
- Impact
- Top 10% most cited in its field
- References
- 37 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Laser Applications in Dentistry and Medicine · Salivary Gland Disorders and Functions · Exercise and Physiological Responses
- Keywords
- CXCL10, Chemokine, Cytokine, Chromatin immunoprecipitation, Monocyte, Cancer research, Flow cytometry, CCL2, Molecular biology, Chemistry, Immunology, Biology, Gene expression, Immune system, Promoter, Gene
- MeSH
- monocytes, cell line, chromatin, mitochondria, humans, inflammation, tumor necrosis factor-alpha, histones, chemokines, cytokines, lasers, gene expression regulation, chemokine ccl2, chemokine cxcl10, promoter regions, genetic, low-level light therapy
8 authors
From TW
- Chia‐Hsin ChenKaohsiung Medical University; Kaohsiung Medical University Chung-Ho Memorial Hospital; Kaohsiung Municipal Ta-Tung Hospital
- Chau‐Zen WangKaohsiung Medical University
- Yan‐Hsiung WangKaohsiung Medical University
- Wei‐Ting LiaoKaohsiung Medical University
- Yi‐Jen ChenKaohsiung Medical University; Kaohsiung Medical University Chung-Ho Memorial Hospital
- Chang‐Hung KuoKaohsiung Medical University; Kaohsiung Medical University Chung-Ho Memorial Hospital
Abstract
Low-level laser therapy (LLLT) has been used in the treatment of radiotherapy-induced oral mucositis and allergic rhinitis. However, the effects of LLLT on human monocyte polarization into M1 macrophages are unknown. To evaluate the effects of LLLT on M1-related cytokine and chemokine production and elucidate the mechanism, the human monocyte cell line THP-1 was treated with different doses of LLLT. The expression of M1-related cytokines and chemokines (CCL2, CXCL10, and TNF-α) was determined by ELISA and real-time PCR. LLLT-associated histone modifications were examined by chromatin immunoprecipitation (ChIP) assays. Mitochondrial involvement in the LLLT-induced M1-related cytokine expression was evaluated by quantitative real-time PCR. Flow cytometry was used to detect the cell surface markers for monocyte polarization. The results showed that LLLT (660 nm) significantly enhanced M1-related cytokine and chemokine expression in mRNA and protein levels. Mitochondrial copy number and mRNA levels of complex I-V protein were increased by LLLT (1 J/cm(2)). Activation of M1 polarization was concomitant with histone modification at TNF-α gene locus and IP-10 gene promoter area. This study indicates that LLLT (660 nm) enhanced M1-related cytokine and chemokine expression via mitochondrial biogenesis and histone modification, which may be a potent immune-enhancing agent for the treatment of allergic diseases.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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