Low-level laser irradiation induces in vitro proliferation of mesenchymal stem cells
Barboza CA, Ginani F, Soares DM, Henriques AC, Freitas Rde A
Einstein (Sao Paulo, Brazil) · 49 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
Based on full-text disclosure statement.
Publication
- Published
- 2014-03-01 · Einstein (Sao Paulo) · vol. 12 · issue 1 · pp. 75–81
- Publisher
- Instituto Israelita de Ensino e Pesquisa Albert Einstein
- Cited
- 70 citations · more than 96% of similar papers · 5.9× the field average
- Impact
- Top 10% most cited in its field
- References
- 28 works
- Access
- Open access (free to publish) · CC-BY
- Research areas
- Laser Applications in Dentistry and Medicine · Periodontal Regeneration and Treatments · Dermatologic Treatments and Research
- Keywords
- Mesenchymal stem cell, Stem cell, DAPI, Adipose tissue, Viability assay, Chemistry, Cell growth, Bone marrow, Cell biology, Cell, Biology, Immunology, Apoptosis, Biochemistry
- MeSH
- adipose tissue, bone marrow cells, cells, cultured, mesenchymal stem cells, animals, humans, mice, reproducibility of results, radiation dosage, dose-response relationship, radiation, cell proliferation, cell survival, time factors, male, lasers, semiconductor, low-level light therapy
5 authors
From BR
- Carlos Augusto Galvão BarbozaUniversidade Federal do Rio Grande do Norte
- Fernanda GinaniUniversidade Federal do Rio Grande do Norte
- Diego Moura SoaresUniversidade Federal de Pernambuco
- Águida Cristina Gomes HenriquesUniversidade Federal do Rio Grande do Norte
- Roseana de Almeida FreitasUniversidade Federal do Rio Grande do Norte
Abstract
Objective
To evaluate the effect of low-level laser irradiation on the proliferation and possible nuclear morphological changes of mouse mesenchymal stem cells.
Methods
Mesenchymal stem cells derived from bone marrow and adipose tissue were submitted to two applications (T0 and T48 hours) of low-level laser irradiation (660 nm; doses of 0.5 and 1.0 J/cm2). The trypan blue assay was used to evaluate cell viability, and growth curves were used to analyze proliferation at zero, 24, 48, and 72 hours. Nuclear alterations were evaluated by staining with DAPI (4'-6-diamidino-2-phenylindole) at 72 hours.
Results
Bone marrow-derived mesenchymal stem cells responded to laser therapy in a dose-dependent manner. Higher cell growth was observed when the cells were irradiated with a dose of 1.0 J/cm2, especially after 24 hours (p<0.01). Adipose-derived mesenchymal stem cells responded better to a dose of 1.0 J/cm2, but higher cell proliferation was observed after 48 hours (p<0.05) and 72 hours (p<0.01). Neither nuclear alterations nor a significant change in cell viability was detected in the studied groups.
Conclusion
Low-level laser irradiation stimulated the proliferation of mouse mesenchymal stem cells without causing nuclear alterations. The biostimulation of mesenchymal stem cells using laser therapy might be an important tool for regenerative therapy and tissue engineering.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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