Near-infrared low-level laser stimulation of telocytes from human myometrium
Campeanu RA, Radu BM, Cretoiu SM, Banciu DD, Banciu A, Cretoiu D, Popescu LM
Lasers in medical science · 41 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- Autoritatea Natională pentru Cercetare Stiintifică
- Government
- Unitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii, Dezvoltarii si Inovarii
- Grants
- Autoritatea Natională pentru Cercetare Stiintifică (82/2012); Unitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii, Dezvoltarii si Inovarii (PN-II-PT-PCCA-2011-3)
Based on 2 listed funder(s).
Publication
- Published
- 2014-05-28 · Lasers Med Sci · vol. 29 · issue 6 · pp. 1867–1874
- Publisher
- Springer Science+Business Media
- Cited
- 60 citations · more than 95% of similar papers · 4.1× the field average
- Impact
- Top 10% most cited in its field
- References
- 41 works
- Access
- Open access (hybrid journal) · CC-BY
- Research areas
- Genetics, Aging, and Longevity in Model Organisms · Laser Applications in Dentistry and Medicine · Planarian Biology and Electrostimulation
- Keywords
- Myometrium, Stimulation, Internal medicine, Endocrinology, Andrology, Chemistry, Medicine, Uterus
- MeSH
- myometrium, uterus, humans, neodymium, receptor, platelet-derived growth factor alpha, antigens, cd34, biopsy, hysterectomy, pregnancy, adult, middle aged, female, lasers, solid-state, low-level light therapy, telocytes
7 authors
From RO, IT
- Razvan-Alexandru CampeanuUniversity of Bucharest
- Beatrice Mihaela RaduUniversity of Verona; University of Bucharest
- Sanda Maria CreţoiuCarol Davila University of Medicine and Pharmacy; Institutul National Victor Babes
- Daniel Dumitru BanciuUniversity of Bucharest
- Adela BanciuUniversity of Bucharest
- Dragoş CrețoiuCarol Davila University of Medicine and Pharmacy; Institutul National Victor Babes
Abstract
Telocytes (TCs) are a brand-new cell type frequently observed in the interstitial space of many organs (see www.telocytes.com ). TCs are defined by very long (tens of micrometers) and slender prolongations named telopodes. At their level, dilations-called podoms (~300 nm), alternate with podomers (80-100 nm). TCs were identified in a myometrial interstitial cell culture based on morphological criteria and by CD34 and PDGF receptor alpha (PDGFRα) immunopositivity. However, the mechanism(s) of telopodes formation and/or elongation and ramification is not known. We report here the low-level laser stimulation (LLLS) using a 1,064-nm neodymium-doped yttrium aluminum garnet (Nd:YAG) laser (with an output power of 60 mW) of the telopodal lateral extension (TLE) growth in cell culture. LLLS of TCs determines a higher growth rate of TLE in pregnant myometrium primary cultures (10.3 ± 1.0 μm/min) compared to nonpregnant ones (6.6 ± 0.9 μm/min). Acute exposure (30 min) of TCs from pregnant myometrium to 1 μM mibefradil, a selective inhibitor of T-type calcium channels, determines a significant reduction in the LLLS TLE growth rate (5.7 ± 0.8 μm/min) compared to LLLS per se in same type of samples. Meanwhile, chronic exposure (24 h) completely abolishes the LLLS TLE growth in both nonpregnant and pregnant myometria. The initial direction of TLE growth was modified by LLLS, the angle of deviation being more accentuated in TCs from human pregnant myometrium than in TCs from nonpregnant myometrium. In conclusion, TCs from pregnant myometrium are more susceptible of reacting to LLLS than those from nonpregnant myometrium. Therefore, some implications are emerging for low-level laser therapy (LLLT) in uterine regenerative medicine.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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