Sulforaphane, quercetin and catechins complement each other in elimination of advanced pancreatic cancer by miR-let-7 induction and K-ras inhibition
Appari M, Babu KR, Kaczorowski A, Gross W, Herr I
International journal of oncology · 113 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- Deutsche Forschungsgemeinschaft
- Nonprofit
- German-Israeli Foundation for Scientific Research and Development
- Nonprofit
- Deutsche Krebshilfe
- Nonprofit
- Heidelberger Stiftung Chirurgie
- Grants
- Deutsche Krebshilfe (109362)
Based on 4 listed funder(s).
Publication
- Published
- 2014-07-08 · Int J Oncol · vol. 45 · issue 4 · pp. 1391–1400
- Publisher
- Spandidos Publishing
- Cited
- 153 citations · more than 98% of similar papers · 6.3× the field average
- Impact
- Top 10% most cited in its field
- References
- 45 works
- Access
- Open access (hybrid journal) · CC-BY-NC
- Research areas
- Genomics, phytochemicals, and oxidative stress · MicroRNA in disease regulation · Curcumin's Biomedical Applications
- Keywords
- Sulforaphane, Pancreatic cancer, Quercetin, Oncogene, Cancer research, Apoptosis, Catechin, Biology, Cancer stem cell, Cancer, Cancer cell, Pharmacology, Cell cycle, Stem cell, Chemistry, Biochemistry, Cell biology, Polyphenol, Antioxidant
- MeSH
- cell line, tumor, humans, carcinoma, pancreatic ductal, pancreatic neoplasms, isothiocyanates, sulfoxides, catechin, quercetin, ras proteins, proto-oncogene proteins, micrornas, plant extracts, antineoplastic agents, antineoplastic combined chemotherapy protocols, cell movement, gene expression regulation, neoplastic, drug synergism, proto-oncogene proteins p21(ras), neoplastic stem cells
5 authors
From DE
- Mahesh AppariGerman Cancer Research Center; Heidelberg University; University Hospital Heidelberg
- Kamesh R. BabuHeidelberg University
- Adam KaczorowskiGerman Cancer Research Center; Heidelberg University; University Hospital Heidelberg
- WOLFGANG GROSGerman Cancer Research Center; Heidelberg University; University Hospital Heidelberg
- INGRID HERGerman Cancer Research Center; Heidelberg University; University Hospital Heidelberg
Abstract
Pancreatic ductal adenocarcinoma (PDA) has the worst prognosis of all malignancies, and current therapeutic options do not target cancer stem cells (CSCs), which may be the reason for the extreme aggressiveness. The dietary agents sulforaphane and quercetin enriched e.g., in broccoli, and the main and best studied green tea catechin EGCG hold promise as anti-CSC agents in PDA. We examined the efficacy of additional catechins and the combination of these bioactive agents to stem cell features and miRNA signaling. Two established and one primary PDA cell line and non-malignant pancreatic ductal cells were used. Whereas each agent strongly inhibited colony formation, the catechins ECG and CG were more effective than EGCG. A mixture of green tea catechins (GTCs) significantly inhibited viability, migration, expression of MMP-2 and -9, ALDH1 activity, colony and spheroid formation and induced apoptosis, but the combination of GTCs with sulforaphane or quercetin was superior. Following treatment with bioactive agents, the expression of miR-let7-a was specifically induced in cancer cells but not in normal cells and it was associated with K-ras inhibition. These data demonstrate that sulforaphane, quercetin and GTC complement each other in inhibition of PDA progression by induction of miR-let7-a and inhibition of K-ras.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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