Low-level laser irradiation promotes the proliferation and maturation of keratinocytes during epithelial wound repair
Sperandio FF, Simões A, Corrêa L, Aranha AC, Giudice FS, Hamblin MR, Sousa SC
Journal of biophotonics · 59 citations
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- People, plus animal or lab work
- Main outcome
- Health markers and function
Who paid for it
- Funding
- Independent funding
- Government
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Government
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Government
- National Institute of Allergy and Infectious Diseases
- Government
- NIAID NIH HHS
- Government
- US NIH
- Government
- CAPES
- Government
- FAPESP
- Grants
- National Institute of Allergy and Infectious Diseases (R01 AI050875)
Based on 7 listed funder(s).
Publication
- Published
- 2014-11-20 · J Biophotonics · vol. 8 · issue 10 · pp. 795–803
- Publisher
- Wiley
- Cited
- 86 citations · more than 97% of similar papers · 5.8× the field average
- Impact
- Top 10% most cited in its field
- References
- 52 works
- Access
- Open access (repository copy)
- Research areas
- Laser Applications in Dentistry and Medicine · Dermatologic Treatments and Research · Marine Invertebrate Physiology and Ecology
- Keywords
- DAPI, HaCaT, Cytokeratin, Wound healing, Cyclin D1, Staining, Keratinocyte, Cell biology, Epithelium, Cell growth, Chemistry, Biology, In vitro, Molecular biology, Immunohistochemistry, Pathology, Cell cycle, Cell, Immunology, Medicine, Biochemistry
- MeSH
- epithelium, cell line, keratinocytes, animals, humans, rats, rats, wistar, wound healing, cell differentiation, cell proliferation, cell movement, female, keratins, low-level light therapy
7 authors
From US, BR
- Felipe Fornias Sperandio · correspondingHarvard University; Universidade Federal de Alfenas; Massachusetts General Hospital
- Alyne SimõesUniversidade de São Paulo
- Luciana Roberta do Vale CorrêaUniversidade de São Paulo
- Ana Cecília Corrêa AranhaUniversidade de São Paulo
- Fernanda Salgueiredo GiudiceAC Camargo Hospital
- Michael R. HamblinHarvard University; Massachusetts General Hospital; Harvard–MIT Division of Health Sciences and Technology
Abstract
Low-level laser therapy (LLLT) has been extensively employed to improve epithelial wound healing, though the exact response of epithelium maturation and stratification after LLLT is unknown. Thus, this study aimed to assess the in vitro growth and differentiation of keratinocytes (KCs) and in vivo wound healing response when treated with LLLT. Human KCs (HaCaT cells) showed an enhanced proliferation with all the employed laser energy densities (3, 6 and 12 J/cm(2) , 660 nm, 100 mW), together with an increased expression of Cyclin D1. Moreover, the immunoexpression of proteins related to epithelial proliferation and maturation (p63, CK10, CK14) all indicated a faster maturation of the migrating KCs in the LLLT-treated wounds. In that way, an improved epithelial healing was promoted by LLLT with the employed parameters; this improvement was confirmed by changes in the expression of several proteins related to epithelial proliferation and maturation. Immunofluorescent expression of cytokeratin 10 (red) and Cyclin D1 (green) in (A) Control keratinocytes and (B) Low-level laser irradiated cells. Blue color illustrates the nuclei of the cells (DAPI staining).
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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