Absorption and chemopreventive targets of sulforaphane in humans following consumption of broccoli sprouts or a myrosinase-treated broccoli sprout extract
Atwell LL, Hsu A, Wong CP, Stevens JF, Bella D, Yu TW, Pereira CB, Löhr CV, Christensen JM, Dashwood RH, Williams DE, Shannon J, Ho E
Molecular nutrition & food research · 104 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Randomized controlled trial (indexed by PubMed)
- Studied in
- People
- Main outcome
- Mechanisms only
- Intake measured by
- Food provided by researchers
Who paid for it
- Funding
- Independent funding
- Government
- National Cancer Institute
- Government
- National Institute of Environmental Health Sciences
- Government
- NCI NIH HHS
- Government
- National Institute of Health - National Cancer Institute
- Government
- NIEHS NIH HHS
- Grants
- National Cancer Institute (R01 CA-122906); National Cancer Institute (CA 090890); National Institute of Environmental Health Sciences (P30ES000210); National Cancer Institute (P01 CA090890); National Cancer Institute (CA122906)
Based on 5 listed funder(s).
Publication
- Published
- 2014-12-17 · Mol Nutr Food Res · vol. 59 · issue 3 · pp. 424–433
- Publisher
- Wiley
- Cited
- 135 citations · more than 94% of similar papers · 3.6× the field average
- Impact
- Top 10% most cited in its field
- References
- 39 works
- Access
- Open access (repository copy)
- Research areas
- Genomics, phytochemicals, and oxidative stress · Curcumin's Biomedical Applications · Phytochemicals and Antioxidant Activities
- Keywords
- Population, BELLA, Sulforaphane, Library science, Traditional medicine, Medicine, Biology, Botany, Environmental health
- MeSH
- humans, brassica, isothiocyanates, sulfoxides, histone deacetylases, glycoside hydrolases, plant extracts, anticarcinogenic agents, gene expression regulation, intestinal absorption, dietary supplements, adult, middle aged, heme oxygenase-1, young adult, molecular targeted therapy
13 authors
From US
- Lauren L. AtwellOregon State University
- Anna J. C. HsuOregon State University
- Carmen P. WongOregon State University
- Jan Frederik StevensOregon State University
- Deborah L. BellaOregon State University
- Tianwei YuOregon State University
Abstract
Scope
Sulforaphane (SFN), an isothiocyanate derived from crucifers, has numerous health benefits. SFN bioavailability from dietary sources is a critical determinant of its efficacy in humans. A key factor in SFN absorption is the release of SFN from its glucosinolate precursor, glucoraphanin, by myrosinase. Dietary supplements are used in clinical trials to deliver consistent SFN doses, but myrosinase is often inactivated in available supplements. We evaluated SFN absorption from a myrosinase-treated broccoli sprout extract (BSE) and are the first to report effects of twice daily, oral dosing on SFN exposure in healthy adults.
Methods and results
Subjects consumed fresh broccoli sprouts or the BSE, each providing 200 μmol SFN daily, as a single dose and as two 100-μmol doses taken 12 h apart. Using HPLC-MS/MS, we detected ∼3 x higher SFN metabolite levels in plasma and urine of sprout consumers, indicating enhanced SFN absorption from sprouts. Twelve-hour dosing retained higher plasma SFN metabolite levels at later time points than 24-hour dosing. No dose responses were observed for molecular targets of SFN (i.e. heme oxygenase-1, histone deacetylase activity, p21).
Conclusion
We conclude that the dietary form and dosing schedule of SFN may impact SFN absorption and efficacy in human trials.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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