Transcriptome analysis reveals in vitro cultured Withania somnifera leaf and root tissues as a promising source for targeted withanolide biosynthesis
Senthil K, Jayakodi M, Thirugnanasambantham P, Lee SC, Duraisamy P, Purushotham PM, Rajasekaran K, Nancy Charles S, Mariam Roy I, Nagappan AK, Kim GS, Lee YS, Natesan S, Min TS, Yang TJ
BMC genomics · 20 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- University Grants Commission
- Government
- Rural Development Administration
Based on 2 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2015-01-21 · BMC Genomics · vol. 16 · issue 1 · p. 14
- Publisher
- BioMed Central
- Cited
- 61 citations · more than 100% of similar papers · 24.5× the field average
- Impact
- Top 10% most cited in its field
- References
- 80 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Phytochemicals and Medicinal Plants · Plant tissue culture and regeneration · Andrographolide Research and Applications
- Keywords
- Withanolide, Withania somnifera, Withaferin A, Biology, Transcriptome, UniGene, KEGG, Biochemistry, Gene expression, Gene
- MeSH
- cell line, tumor, humans, withania, plant leaves, plant roots, antioxidants, sequence analysis, rna, cell proliferation, cell survival, microsatellite repeats, expressed sequence tags, withanolides, transcriptome, cell cycle checkpoints
15 authors
From IN, KR
- Kalaiselvi SenthilAvinashilingam University
- Murukarthick JayakodiSeoul National University
- Pankajavalli ThirugnanasambanthamAvinashilingam University
- Sang Choon LeeSeoul National University
- Pradeepa DuraisamyAvinashilingam University
- Preethi M. PurushothamAvinashilingam University
Abstract
Background
The production of metabolites via in vitro culture is promoted by the availability of fully defined metabolic pathways. Withanolides, the major bioactive phytochemicals of Withania somnifera, have been well studied for their pharmacological activities. However, only a few attempts have been made to identify key candidate genes involved in withanolide biosynthesis. Understanding the steps involved in withanolide biosynthesis is essential for metabolic engineering of this plant to increase withanolide production.
Results
Transcriptome sequencing was performed on in vitro adventitious root and leaf tissues using the Illumina platform. We obtained a total of 177,156 assembled transcripts with an average unigene length of 1,033 bp. About 13% of the transcripts were unique to in vitro adventitious roots but no unique transcripts were observed in in vitro-grown leaves. A putative withanolide biosynthetic pathway was deduced by mapping the assembled transcripts to the KEGG database, and the expression of candidate withanolide biosynthesis genes -were validated by qRT PCR. The accumulation pattern of withaferin A and withanolide A varied according to the type of tissue and the culture period. Further, we demonstrated that in vitro leaf extracts exhibit anticancer activity against human gastric adenocarcinoma cell lines at sub G1 phase.
Conclusions
We report here a validated large-scale transcriptome data set and the potential biological activity of in vitro cultures of W. somnifera. This study provides important information to enhance tissue-specific expression and accumulation of secondary metabolites, paving the way for industrialization of in vitro cultures of W. somnifera.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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