Antiproliferative and antioxidant properties of anthocyanin rich extracts from blueberry and blackcurrant juice
Diaconeasa Z, Leopold L, Rugină D, Ayvaz H, Socaciu C
International journal of molecular sciences · 99 citations
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- No health outcome
Who paid for it
- Funding
- Independent funding
- Government
- Autoritatea Natională pentru Cercetare Stiintifică
- Government
- Unitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii, Dezvoltarii si Inovarii
- Government
- This paper was published under the frame of European Social Fund, Human Resources Development Operational Programme 2007-2013, project no. POSDRU/159/1.5/S/132765 and by a UEFISCDI grant, project number PN-II-RU-PD-2012-3-0283
- University or hospital
- We are also grateful to Dr. Manuela Banciu for providing the B16-F10 murine melanoma cell line and to Chiricuta Cancer Institute, Cluj-Napoca for providing HeLa and A2780 human cancer cell lines
- Grants
- Unitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii, Dezvoltarii si Inovarii (PN-II-RU-PD-2012-3-0283)
Based on 4 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2015-01-22 · Int J Mol Sci · vol. 16 · issue 2 · pp. 2352–2365
- Publisher
- Multidisciplinary Digital Publishing Institute
- Cited
- 215 citations · more than 99% of similar papers · 10.1× the field average
- Impact
- Top 10% most cited in its field
- References
- 70 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Phytochemicals and Antioxidant Activities · Botanical Research and Applications · Natural Antidiabetic Agents Studies
- Keywords
- Anthocyanin, DPPH, Chemistry, Food science, Antioxidant, HeLa, Cyanidin, Flavonoid, Polyphenol, MTT assay, Vaccinium, Delphinidin, ABTS, Traditional medicine, Biochemistry, Botany, Biology, Cell growth, In vitro, Medicine
- MeSH
- cell line, tumor, hela cells, ht29 cells, animals, humans, mice, anthocyanins, plant extracts, antineoplastic agents, phytogenic, antioxidants, chromatography, high pressure liquid, spectrometry, mass, electrospray ionization, cell proliferation, cell survival, beverages, solid phase extraction, blueberry plants
5 authors
From RO, TR
- Zorița Maria DiaconeasaUniversity of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca
- Loredana Florina LEOPOLDUniversity of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca
- Dumitriţa RuginǎUniversity of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca
- Hüseyin AyvazÇanakkale Onsekiz Mart Üniversitesi
- Carmen SocaciuUniversity of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca
Abstract
The present study was aimed at evaluating the antiproliferative potential of anthocyanin-rich fractions (ARFs) obtained from two commercially available juices (blueberry and blackcurrant juices) on three tumor cell lines; B16F10 (murine melanoma), A2780 (ovarian cancer) and HeLa (cervical cancer). Individual anthocyanin determination, identification and quantification were done using HPLC-MS. Antioxidant activity of the juices was determined through different mechanism methods such as DPPH and ORAC. For biological testing, the juices were purified through C18 cartridges in order to obtain fractions rich in anthocyanins. The major anthocyanins identified were glycosylated cyanidin derivatives. The antiproliferative activity of the fractions was tested using the MTT assay. The antiproliferative potential of ARF was found to be associated with those bioactive molecules, anthocyanins due to their antioxidant potential. The results obtained indicated that both blueberry and blackcurrants are rich sources of antioxidants including anthocyanins and therefore these fruits are highly recommended for daily consumption to prevent numerous degenerative diseases.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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