Biomodulation of Inflammatory Cytokines Related to Oral Mucositis by Low-Level Laser Therapy
Basso FG, Pansani TN, Soares DG, Scheffel DL, Bagnato VS, de Souza Costa CA, Hebling J
Photochemistry and photobiology · 43 citations
Review labels
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How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Government
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Grants
- Fundação de Amparo à Pesquisa do Estado de São Paulo (2014/06057‐7); Conselho Nacional de Desenvolvimento Científico e Tecnológico (301029/2010-1); Fundação de Amparo à Pesquisa do Estado de São Paulo (2012/17947‐8); Fundação de Amparo à Pesquisa do Estado de São Paulo (13/05879-0)
Based on 2 listed funder(s).
Publication
- Published
- 2015-03-04 · Photochem Photobiol · vol. 91 · issue 4 · pp. 952–956
- Publisher
- Wiley
- Cited
- 62 citations · more than 95% of similar papers · 4.7× the field average
- Impact
- Top 10% most cited in its field
- References
- 34 works
- Access
- Paywalled
- Research areas
- Laser Applications in Dentistry and Medicine · Oral health in cancer treatment · Salivary Gland Disorders and Functions
- Keywords
- Mucositis, Low level laser therapy, Tumor necrosis factor alpha, Interleukin 6, Lipopolysaccharide, Interleukin 8, Proinflammatory cytokine, Chemistry, Protein expression, Gene expression, Molecular biology, Medicine, Inflammation, Andrology, Immunology, Dentistry, Internal medicine, Biology, Gene, Laser therapy, Biochemistry, Radiation therapy, Laser
- MeSH
- humans, stomatitis, inflammation mediators, cytokines, lasers
7 authors
From BR
- Fernanda Gonçalves BassoCentro Universitário de Araraquara; Universidade Estadual Paulista (Unesp)
- Taísa Nogueira PansaniCentro Universitário de Araraquara; Universidade Estadual Paulista (Unesp)
- Diana Gabriela SoaresCentro Universitário de Araraquara; Universidade Estadual Paulista (Unesp)
- Débora Lopes Salles ScheffelCentro Universitário de Araraquara; Universidade Estadual Paulista (Unesp)
- Vanderlei Salvador Bagnato
- Carlos Alberto de Souza CostaCentro Universitário de Araraquara; Universidade Estadual Paulista (Unesp)
Abstract
This study evaluated the effects of LLLT on the expression of inflammatory cytokines related to the development of oral mucositis by gingival fibroblasts. Primary gingival fibroblasts were seeded on 24-well plates (10(5) cells/well) for 24 h. Fresh serum-free culture medium (DMEM) was then added, and cells were placed in contact with LPS (Escherichia coli, 1 μg mL(-1)), followed by LLLT irradiation (LaserTABLE-InGaAsP diode prototype-780 nm, 25 mW) delivering 0, 0.5, 1.5 or 3 J cm(-2)². Cells without contact with LPS were also irradiated with the same energy densities. Gene expression of TNF-α, IL-1β, IL-6 and IL-8 was evaluated by Real-Time PCR, and protein synthesis of these cytokines was determined by enzyme-linked immunosorbent (ELISA) assay. Data were statistically analyzed by the Kruskal-Wallis test, complemented by the Mann-Whitney test (P < 0.05). LPS treatment increased the gene expression and protein synthesis of TNF-α, IL-6 and IL-8, while the expression of IL-1β was not affected. For LPS-treated groups, LLLT promoted significant decreases in the expression of TNF-α, IL-6, and IL-8 at 1.5 J cm(-2) and 3 J cm(-2). These results demonstrate that LLLT promoted a beneficial biomodulatory effect on the expression of inflammatory cytokines related to oral mucositis by human gingival fibroblasts.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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