Combinations of Ashwagandha leaf extracts protect brain-derived cells against oxidative stress and induce differentiation
Shah N, Singh R, Sarangi U, Saxena N, Chaudhary A, Kaur G, Kaul SC, Wadhwa R
PloS one · 35 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- Ministry of Education, Culture, Sports, Science and Technology
Based on 1 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2015-03-19 · PLoS One · vol. 10 · issue 3 · p. e0120554
- Publisher
- Public Library of Science
- Cited
- 69 citations · more than 99% of similar papers · 16.4× the field average
- Impact
- Top 10% most cited in its field
- References
- 47 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Phytochemicals and Medicinal Plants · Medicinal Plants and Neuroprotection · Pharmacology and Nanomedicine Research
- Keywords
- Neuroprotection, Oxidative stress, In vivo, Pharmacology, Biology, In vitro, Chemistry, Traditional medicine, Biochemistry, Medicine, Biotechnology
- MeSH
- cell line, tumor, animals, humans, rats, withania, plant leaves, hydrogen peroxide, glutamic acid, neuroprotective agents, plant extracts, microscopy, fluorescence, apoptosis, cell differentiation, cell survival, oxidative stress
8 authors
From JP, IN
- Navjot ShahNational Institute of Advanced Industrial Science and Technology
- Rumani SinghNational Institute of Advanced Industrial Science and Technology
- Upasana SarangiNational Institute of Advanced Industrial Science and Technology
- Nishant SaxenaNational Institute of Advanced Industrial Science and Technology
- Anupama ChaudharyNational Institute of Advanced Industrial Science and Technology
- Gurcharan KaurGuru Nanak Dev University
Abstract
Background
Ashwagandha, a traditional Indian herb, has been known for its variety of therapeutic activities. We earlier demonstrated anticancer activities in the alcoholic and water extracts of the leaves that were mediated by activation of tumor suppressor functions and oxidative stress in cancer cells. Low doses of these extracts were shown to possess neuroprotective activities in vitro and in vivo assays.
Methodology/principal findings
We used cultured glioblastoma and neuroblastoma cells to examine the effect of extracts (alcoholic and water) as well as their bioactive components for neuroprotective activities against oxidative stress. Various biochemical and imaging assays on the marker proteins of glial and neuronal cells were performed along with their survival profiles in control, stressed and recovered conditions. We found that the extracts and one of the purified components, withanone, when used at a low dose, protected the glial and neuronal cells from oxidative as well as glutamate insult, and induced their differentiation per se. Furthermore, the combinations of extracts and active component were highly potent endorsing the therapeutic merit of the combinational approach.
Conclusion
Ashwagandha leaf derived bioactive compounds have neuroprotective potential and may serve as supplement for brain health.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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