Effect of various concentrations of caffeine, pentoxifylline, and kallikrein on hyperactivation of frozen bovine semen
Barakat IA, Danfour MA, Galewan FA, Dkhil MA
BioMed research international · 23 citations
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Animals
- Main outcome
- Health markers and function
Who paid for it
- Funding
- Independent funding
- University or hospital
- King Saud University
- Grants
- King Saud University (RG–1435–058)
Based on 1 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2015-01-01 · Biomed Res Int · vol. 2015 · pp. 1–7
- Publisher
- Hindawi Publishing Corporation
- Cited
- 47 citations · more than 92% of similar papers · 3.8× the field average
- Impact
- Top 10% most cited in its field
- References
- 62 works
- Access
- Open access (hybrid journal) · CC-BY
- Research areas
- Sperm and Testicular Function · Reproductive Biology and Fertility · Ovarian function and disorders
- Keywords
- Pentoxifylline, Caffeine, Kallikrein, Hyperactivation, Semen, Motility, Sperm motility, Andrology, Sperm, Chemistry, Biology, Internal medicine, Medicine, Endocrinology, Biochemistry, Enzyme
- MeSH
- spermatozoa, animals, cattle, humans, caffeine, pentoxifylline, kallikreins, cryopreservation, semen preservation, sperm motility, fertilization, male
4 authors
From SA, EG, LY
- Ibrahim A. H. Barakat · correspondingKing Saud University; National Research Centre
- Mohamed A. DanfourMisurata University
- Fatma A. M. GalewanMisurata University
- Mohamed A. DkhilKing Saud University; Helwan University
Abstract
Caffeine, pentoxifylline, and kallikrein are substances that affect the efficiency of sperms in the fertilization process; however, they have not been adequately studied. The present study aimed to examine the influence of caffeine, kallikrein, and pentoxifylline on sperm motility in bovine as well as investigate their optimum concentrations for increasing the movement of sperms in bovine. Frozen bovine sperms were thawed in universal IVF medium supplemented with 1, 5, and 10 mM caffeine or pentoxifylline or 1, 4, and 8 U/mL kallikrein and were then incubated for 30 min. Treated semen parameters were analyzed using a computer assisted semen analyzer (CASA). Data analysis showed that the mean values concerning progression and motility of sperm increased in caffeine and pentoxifylline treatments when compared with the kallikrein group. The obtained results revealed that kallikrein is not necessary for the improvement of bovine sperm motility. Additionally, our results revealed that 5 mM from caffeine was the best concentration added to the medium, followed by 1 or 5 mM from pentoxifylline. Therefore, it is concluded from the present study that caffeine has hyperactivation efficacy at 5 mM concentration compared to other treatments.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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