Identification of anti-biofilm components in Withania somnifera and their effect on virulence of Streptococcus mutans biofilms
Pandit S, Cai JN, Song KY, Jeon JG
Journal of applied microbiology · 18 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- University or hospital
- Chonbuk National University Hospital
- University or hospital
- Biomedical Research Institute
Based on 2 listed funder(s).
Publication
- Published
- 2015-05-14 · J Appl Microbiol · vol. 119 · issue 2 · pp. 571–581
- Publisher
- Oxford University Press
- Cited
- 22 citations · more than 84% of similar papers · 2.0× the field average
- References
- 52 works
- Access
- Paywalled
- Research areas
- Oral microbiology and periodontitis research · Phytochemicals and Medicinal Plants · Bacterial biofilms and quorum sensing
- Keywords
- Biofilm, Streptococcus mutans, Withania somnifera, Oleic acid, Microbiology, Chemistry, Linoleic acid, Virulence, Polysaccharide, Bacteria, Biology, Biochemistry, Food science, Fatty acid
- MeSH
- humans, biofilms, streptococcus mutans, withania, streptococcal infections, dental caries, plant extracts, virulence, gas chromatography-mass spectrometry
4 authors
From KR
- Santosh PanditJeonbuk National University
- Jina-Na CaiJeonbuk National University
- Kwang‐Yeob Song · correspondingJeonbuk National University Hospital
- Jae‐Gyu JeonJeonbuk National University Hospital; Jeonbuk National University
Abstract
Aims
The aim of this study was to identify components of the Withania somnifera that could show anti-virulence activity against Streptococcus mutans biofilms.
Methods and results
The anti-acidogenic activity of fractions separated from W. somnifera was compared, and then the most active anti-acidogenic fraction was chemically characterized using gas chromatography-mass spectroscopy. The effect of the identified components on the acidogenicity, aciduricity and extracellular polymeric substances (EPS) formation of S. mutans UA159 biofilms was evaluated. The change in accumulation and acidogenicity of S. mutans UA159 biofilms by periodic treatments (10 min per treatment) with the identified components was also investigated. Of the fractions, n-hexane fraction showed the strongest anti-acidogenic activity and was mainly composed of palmitic, linoleic and oleic acids. Of the identified components, linoleic and oleic acids strongly affected the acid production rate, F-ATPase activity and EPS formation of the biofilms. Periodic treatment with linoleic and oleic acids during biofilm formation also inhibited the biofilm accumulation and acid production rate of the biofilms without killing the biofilm bacteria.
Conclusions
These results suggest that linoleic and oleic acids may be effective agents for restraining virulence of S. mutans biofilms.
Significance and impact of the study
Linoleic and oleic acids may be promising agents for controlling virulence of cariogenic biofilms and subsequent dental caries formation.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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