Additive regulation of adiponectin expression by the mediterranean diet olive oil components oleic Acid and hydroxytyrosol in human adipocytes
Scoditti E, Massaro M, Carluccio MA, Pellegrino M, Wabitsch M, Calabriso N, Storelli C, De Caterina R
PloS one · 51 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
Based on full-text disclosure statement.
Publication
- Published
- 2015-06-01 · PLoS One · vol. 10 · issue 6 · p. e0128218
- Publisher
- Public Library of Science
- Cited
- 78 citations · more than 94% of similar papers · 3.9× the field average
- Impact
- Top 10% most cited in its field
- References
- 62 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Adipokines, Inflammation, and Metabolic Diseases · Adipose Tissue and Metabolism · Antioxidant Activity and Oxidative Stress
- Keywords
- Hydroxytyrosol, Adiponectin, Endocrinology, Internal medicine, Oleic acid, Adipocyte, Chemistry, Tumor necrosis factor alpha, Oxidative stress, Oleuropein, Peroxisome proliferator-activated receptor, Beta oxidation, Adipose tissue, Antioxidant, Receptor, Biology, Medicine, Insulin resistance, Biochemistry, Insulin, Metabolism, Polyphenol
- MeSH
- 3t3-l1 cells, adipocytes, animals, humans, mice, phenylethyl alcohol, jnk mitogen-activated protein kinases, oleic acid, tumor necrosis factor-alpha, ppar gamma, gene expression regulation, down-regulation, enzyme activation, diet, mediterranean, drug synergism, adiponectin, olive oil
8 authors
From IT, DE
- Egeria ScodittiIstituto di Fisiologia Clinica; National Research Council
- Marika MassaroIstituto di Fisiologia Clinica; National Research Council
- Maria Annunziata CarluccioIstituto di Fisiologia Clinica; National Research Council
- Mariangela PellegrinoUniversity of Salento
- Martin WabitschUniversität Ulm
- Nadia CalabrisoIstituto di Fisiologia Clinica; National Research Council
Abstract
Adiponectin, an adipocyte-derived insulin-sensitizing and anti-inflammatory hormone, is suppressed in obesity through mechanisms involving chronic inflammation and oxidative stress. Olive oil consumption is associated with beneficial cardiometabolic actions, with possible contributions from the antioxidant phenol hydroxytyrosol (HT) and the monounsaturated fatty acid oleic acid (OA, 18:1n-9 cis), both possessing anti-inflammatory and vasculo-protective properties. We determined the effects of HT and OA, alone and in combination, on adiponectin expression in human and murine adipocytes under pro-inflammatory conditions induced by the cytokine tumor necrosis factor(TNF)-α. We used human Simpson-Golabi-Behmel syndrome (SGBS) adipocytes and murine 3T3-L1 adipocytes as cell model systems, and pretreated them with 1-100 μmol/L OA, 0.1-20 μmol/L HT or OA plus HT combination before stimulation with 10 ng/mL TNF-α. OA or HT significantly (P<0.05) prevented TNF-α-induced suppression of total adiponectin secretion (by 42% compared with TNF-α alone) as well as mRNA levels (by 30% compared with TNF-α alone). HT and OA also prevented-by 35%-TNF-α-induced downregulation of peroxisome proliferator-activated receptor PPARγ. Co-treatment with HT and OA restored adiponectin and PPARγ expression in an additive manner compared with single treatments. Exploring the activation of JNK, which is crucial for both adiponectin and PPARγ suppression by TNF-α, we found that HT and OA additively attenuated TNF-α-stimulated JNK phosphorylation (up to 55% inhibition). In conclusion, the virgin olive oil components OA and HT, at nutritionally relevant concentrations, have additive effects in preventing adiponectin downregulation in inflamed adipocytes through an attenuation of JNK-mediated PPARγ suppression.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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