The Effect of Creatine Kinase Inhibition on Contractile Properties of Human Resistance Arteries
Taherzadeh Z, Karamat FA, Ankum WM, Clark JF, van Montfrans GA, van Bavel E, Brewster LM
American journal of hypertension · 20 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- People
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek
Based on 1 listed funder(s).
Publication
- Published
- 2015-06-04 · Am J Hypertens · vol. 29 · issue 2 · pp. 170–177
- Publisher
- Oxford University Press
- Cited
- 24 citations · more than 89% of similar papers · 2.4× the field average
- References
- 34 works
- Access
- Open access (repository copy) · OTHER-OA
- Research areas
- Pregnancy and preeclampsia studies · Nitric Oxide and Endothelin Effects · Cardiovascular Health and Disease Prevention
- Keywords
- Contractility, Medicine, Myograph, Internal medicine, Sodium nitroprusside, Endocrinology, Blood pressure, Vascular resistance, Cardiology, Vasodilation, Creatine kinase, Nitric oxide
- MeSH
- arteries, humans, dinitrofluorobenzene, creatine kinase, blood pressure, adult, middle aged, female, in vitro techniques
7 authors
From IR, NL, US
- Zhila TaherzadehMashhad University of Medical Sciences; Amsterdam UMC Location University of Amsterdam; University of Amsterdam
- Fares A. KaramatAmsterdam UMC Location University of Amsterdam; University of Amsterdam
- Willem M. AnkumAmsterdam UMC Location University of Amsterdam; University of Amsterdam
- Joseph F. ClarkUniversity of Cincinnati
- Gert A. van MontfransAmsterdam UMC Location University of Amsterdam; University of Amsterdam
- Ed van BavelAmsterdam UMC Location University of Amsterdam; University of Amsterdam
Abstract
Background
Creatine kinase (CK) is a main predictor of blood pressure, and this is thought to largely depend on high resistance artery contractility. We previously reported an association between vascular contractility and CK in normotensive pregnancy, but pregnancy is a strong CK inducer, and data on human hypertension are lacking. Therefore, we further explored CK-dependency of vascular contractility outside the context of pregnancy in normotensive and hypertensive women.
Methods and results
Nineteen consecutive women, mean age 42 years (SE 1.3), mean systolic/diastolic blood pressure respectively 142.6 (SE 5.9)/85.6 (3.4) mm Hg (9 hypertensive), donated an omental fat sample during abdominal surgery. We compared vasodilation after the specific CK inhibitor 2,4-dinitro-1-fluorobenzene (DNFB; 10(-6) mol/l) to sodium nitroprusside (10(-6) mol/l) in isolated resistance arteries using a wire myograph. Additionally, we assessed predictors of vasoconstrictive force. DNFB reduced vascular contractility to 24.3% (SE 4.4), P < 0.001, compared to baseline. Sodium nitroprusside reduced contractility to 89.8% (SE 2.3). Maximum contractile force correlated with DNFB effect as a measure of CK (r = 0.8), and with vessel diameter (r = 0.7). The increase in contractile force was 16.5 mN [9.1-23.9] per unit DNFB effect in univariable and 10.35 mN [2.10-18.60] in multivariable regression analysis.
Conclusion
This study extends on our previous findings in pregnant normotensive women of CK-dependent microvascular contractility, indicating that CK contributes significantly to resistance artery contractility across human normotension and primary hypertension outside the context of pregnancy. Further studies should explore the effect of CK inhibitors on clinical blood pressure.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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