Withania somnifera Root Extract Has Potent Cytotoxic Effect against Human Malignant Melanoma Cells
Halder B, Singh S, Thakur SS
PloS one · 15 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- Council of Scientific and Industrial Research, India
Based on 1 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2015-09-03 · PLoS One · vol. 10 · issue 9 · p. e0137498
- Publisher
- Public Library of Science
- Cited
- 46 citations · more than 99% of similar papers · 14.7× the field average
- Impact
- Top 10% most cited in its field
- References
- 24 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Phytochemicals and Medicinal Plants · Medicinal Plants and Neuroprotection · Pharmacological Effects of Medicinal Plants
- Keywords
- Withania somnifera, Cytotoxicity, DAPI, Traditional medicine, MTT assay, Cytotoxic T cell, Pharmacology, Chemistry, Biology, Cell, In vitro, Apoptosis, Pathology, Medicine, Biochemistry
- MeSH
- cell line, tumor, humans, withania, plant roots, melanoma, plant extracts, medicine, ayurvedic, phytotherapy, dna fragmentation, cell survival
3 authors
From IN
- Babli HalderCentre for Cellular and Molecular Biology
- Shruti SinghCentre for Cellular and Molecular Biology
- Suman Siddharth Thakur · correspondingCentre for Cellular and Molecular Biology
Abstract
In Ayurveda, Withania somnifera is commonly known as Ashwagandha, its roots are specifically used in medicinal and clinical applications. It possesses numerous therapeutic actions which include anti-inflammatory, sedative, hypnotic and narcotic. Extracts from this plant have been reported for its anticancer properties. In this study we evaluated for the first time, the cytotoxic effect of Withania root extract on human malignant melanoma A375 cells. The crude extract of Withania was tested for cytotoxicity against A375 cells by MTT assay. Cell morphology of treated A375 cells was visualized through phase contrast as well as fluorescence microscopy. Agarose gel electrophoresis was used to check DNA fragmentation of the crude extract treated cells. Crude extract of Withania root has the potency to reduce viable cell count in dose as well as time dependent manner. Morphological change of the A375 cells was also observed in treated groups in comparison to untreated or vehicle treated control. Apoptotic body and nuclear blebbing were observed in DAPI stained treated cells under fluorescence microscope. A ladder of fragmented DNA was noticed in treated cells. Thus it might be said that the crude water extract of Withania somnifera has potent cytotoxic effect on human malignant melanoma A375 cells.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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