Effect of Whole-Grain Barley on the Human Fecal Microbiota and Metabolome
De Angelis M, Montemurno E, Vannini L, Cosola C, Cavallo N, Gozzi G, Maranzano V, Di Cagno R, Gobbetti M, Gesualdo L
Applied and environmental microbiology · 110 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Controlled clinical trial (classified by our AI screen)
- Studied in
- People
- Main outcome
- Mechanisms only
- Intake measured by
- Not stated
Who paid for it
- Funding
- Funding not disclosed
Publication
- Published
- 2015-09-19 · Appl Environ Microbiol · vol. 81 · issue 22 · pp. 7945–7956
- Publisher
- American Society for Microbiology
- Cited
- 150 citations · more than 97% of similar papers · 6.4× the field average
- Impact
- Top 10% most cited in its field
- References
- 66 works
- Access
- Free to read
- Research areas
- Gut microbiota and health · Probiotics and Fermented Foods · Diet and metabolism studies
- Keywords
- Metabolome, Fusobacteria, Biology, Ruminococcus, Clostridium, Microbiology, Food science, Prevotella, Firmicutes, Feces, Clostridiaceae, Bacteroides, Clostridia, Bacteroidaceae, Bacteria, Metabolite, Biochemistry, 16S ribosomal RNA
- MeSH
- feces, humans, bacteria, hordeum, triticum, rna, ribosomal, 16s, diet, sequence analysis, dna, phylogeny, adult, middle aged, italy, female, male, gas chromatography-mass spectrometry, solid phase microextraction, metabolome, microbiota
10 authors
From IT
- Maria De AngelisUniversity of Bari Aldo Moro
- Eustacchio MontemurnoUniversity of Bari Aldo Moro
- Lucia VanniniResearch Centre for Cereal and Industrial Crops; University of Bologna
- Carmela CosolaUniversity of Bari Aldo Moro
- Noemi CavalloUniversity of Bari Aldo Moro
- Giorgia GozziUniversity of Bologna
Abstract
In this study, we compared the fecal microbiota and metabolomes of 26 healthy subjects before (HS) and after (HSB) 2 months of diet intervention based on the administration of durum wheat flour and whole-grain barley pasta containing the minimum recommended daily intake (3 g) of barley β-glucans. Metabolically active bacteria were analyzed through pyrosequencing of the 16S rRNA gene and community-level catabolic profiles. Pyrosequencing data showed that levels of Clostridiaceae (Clostridium orbiscindens and Clostridium sp.), Roseburia hominis, and Ruminococcus sp. increased, while levels of other Firmicutes and Fusobacteria decreased, from the HSB samples to the HS fecal samples. Community-level catabolic profiles were lower in HSB samples. Compared to the results for HS samples, cultivable lactobacilli increased in HSB fecal samples, while the numbers of Enterobacteriaceae, total coliforms, and Bacteroides, Porphyromonas, Prevotella, Pseudomonas, Alcaligenes, and Aeromonas bacteria decreased. Metabolome analyses were performed using an amino acid analyzer and gas chromatography-mass spectrometry solid-phase microextraction. A marked increase in short-chain fatty acids (SCFA), such as 2-methyl-propanoic, acetic, butyric, and propionic acids, was found in HSB samples with respect to the HS fecal samples. Durum wheat flour and whole-grain barley pasta containing 3% barley β-glucans appeared to be effective in modulating the composition and metabolic pathways of the intestinal microbiota, leading to an increased level of SCFA in the HSB samples.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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