Pterostilbine, an active component of blueberries, sensitizes colon cancer cells to 5-fluorouracil cytotoxicity
Tolba MF, Abdel-Rahman SZ
Scientific reports · 42 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Funding not disclosed
Publication
- Published
- 2015-10-16 · Sci Rep · vol. 5 · issue 1 · p. 15239
- Publisher
- Nature Portfolio
- Cited
- 55 citations · more than 91% of similar papers · 2.7× the field average
- Impact
- Top 10% most cited in its field
- References
- 55 works
- Access
- Open access (journal) · CC-BY
- Research areas
- FOXO transcription factor regulation · Plant tissue culture and regeneration · PI3K/AKT/mTOR signaling in cancer
- Keywords
- Colorectal cancer, Cytotoxic T cell, Caco-2, Cytotoxicity, Cancer research, Apoptosis, Fluorouracil, Cancer cell, Toxicity, Chemistry, Protein kinase B, Pharmacology, Medicine, Cancer, Internal medicine, Cell, Biochemistry, In vitro
- MeSH
- caco-2 cells, hct116 cells, humans, colonic neoplasms, stilbenes, fluorouracil, mitogen-activated protein kinase 1, mitogen-activated protein kinase 3, estrogen receptor beta, antineoplastic agents, apoptosis, binding sites, catalytic domain, phosphorylation, drug synergism, cyclin-dependent kinase inhibitor p27, proto-oncogene proteins c-akt, forkhead transcription factors, cell cycle checkpoints, molecular docking simulation, blueberry plants, forkhead box protein o1
2 authors
From EG, US
- Mai Fathy TolbaAin Shams University; American University in Cairo
- Sherif Z. Abdel‐RahmanThe University of Texas Medical Branch at Galveston
Abstract
Although colorectal cancer (CRC) treatment with 5-fluorouracil (5-FU) is the first line of therapy for this debilitating disease, treatment effectiveness is often hampered by the development of drug resistance and toxicity at high doses. ER-β can play an important role in CRC development and possibly in its response to therapy. Pterostilbene (PT) possesses antioxidant and anticancer effects that are mediated by ER-β. In the current study, we test the hypothesis that PT sensitizes colon cancer cells to 5-FU and we examine the underlying mechanism(s) by which PT exerts its cytotoxic effects in CRC cells. Our data indicate that PT exhibited a more potent cytotoxic effect in Caco-2 compared to HCT-116 cells. PT/5-FU co-treatment was more effective in Caco-2 cells. Our data indicate that ER-β is expressed at higher levels in Caco-2 cells and its levels are further boosted with PT treatment. PT significantly suppressed Akt and ERK phosphorylations, and enhanced FOXO-1 and p27(kip1) levels in Caco-2 cells. PT also induced a significant increase in Caco-2 cells at pre-G phase coupled with increased Bax/Bcl-2 ratio and PARP cleavage. These results provide a rationale for novel combination treatment strategies, especially for patients with 5-FU-resistant tumors expressing ER-β protein.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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