Sulforaphane inhibits hypoxia-induced HIF-1α and VEGF expression and migration of human colon cancer cells
Kim DH, Sung B, Kang YJ, Hwang SY, Kim MJ, Yoon JH, Im E, Kim ND
International journal of oncology · 78 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- University or hospital
- Pusan National University
Based on 1 listed funder(s).
Publication
- Published
- 2015-10-13 · Int J Oncol · vol. 47 · issue 6 · pp. 2226–2232
- Publisher
- Spandidos Publishing
- Cited
- 94 citations · more than 92% of similar papers · 3.1× the field average
- Impact
- Top 10% most cited in its field
- References
- 41 works
- Access
- Free to read
- Research areas
- Genomics, phytochemicals, and oxidative stress · Cancer, Hypoxia, and Metabolism · Eicosanoids and Hypertension Pharmacology
- Keywords
- Sulforaphane, Cancer research, Hypoxia-inducible factors, Protein kinase B, Vascular endothelial growth factor, Angiogenesis, Biology, Cancer cell, Hypoxia (environmental), Apoptosis, Cancer, Chemistry, Biochemistry, VEGF receptors
- MeSH
- hct116 cells, humans, colonic neoplasms, isothiocyanates, sulfoxides, vascular endothelial growth factor a, anticarcinogenic agents, blotting, western, enzyme-linked immunosorbent assay, cell movement, cell hypoxia, hypoxia-inducible factor 1, alpha subunit
8 authors
From KR
- Dong Hwan KimPusan National University
- Bokyung SungPusan National University
- Yong Jung KangPusan National University
- Seong Yeon HwangPusan National University
- Min Jeong KimPusan National University
- Jeong-Hyun YoonPusan National University
Abstract
The effects of sulforaphane (a natural product commonly found in broccoli) was investigated on hypoxia inducible factor-1α (HIF-1α) expression in HCT116 human colon cancer cells and AGS human gastric cancer cells. We found that hypoxia-induced HIF-1α protein expression in HCT116 and AGS cells, while treatment with sulforaphane markedly and concentration-dependently inhibited HIF-1α expression in both cell lines. Treatment with sulforaphane inhibited hypoxia-induced vascular endothelial growth factor (VEGF) expression in HCT116 cells. Treatment with sulforaphane modulated the effect of hypoxia on HIF-1α stability. However, degradation of HIF-1α by sulforaphane was not mediated through the 26S proteasome pathway. We also found that the inhibition of HIF-1α by sulforaphane was not mediated through AKT and extracellular signal-regulated kinase phosphorylation under hypoxic conditions. Finally, hypoxia-induced HCT116 cell migration was inhibited by sulforaphane. These data suggest that sulforaphane may inhibit human colon cancer progression and cancer cell angiogenesis by inhibiting HIF-1α and VEGF expression. Taken together, these results indicate that sulforaphane is a new and potent chemopreventive drug candidate for treating patients with human colon cancer.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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