Amelioration of Isoproterenol-Induced Oxidative Damage in Rat Myocardium by Withania somnifera Leaf Extract
Khalil MI, Ahmmed I, Ahmed R, Tanvir EM, Afroz R, Paul S, Gan SH, Alam N
BioMed research international · 74 citations
How it was studied
- Design
- Animal study (classified by our AI screen)
- Studied in
- Animals
- Main outcome
- Health markers and function
Who paid for it
- Funding
- Independent funding
Based on full-text disclosure statement.
Publication
- Published
- 2015-01-01 · Biomed Res Int · vol. 2015 · pp. 1–10
- Publisher
- Hindawi Publishing Corporation
- Cited
- 110 citations · more than 99% of similar papers · 19.7× the field average
- Impact
- Top 10% most cited in its field
- References
- 49 works
- Access
- Open access (hybrid journal) · CC-BY
- Research areas
- Phytochemicals and Medicinal Plants · Cardiac Ischemia and Reperfusion · Medicinal Plants and Neuroprotection
- Keywords
- Withania somnifera, Lipid peroxidation, Glutathione peroxidase, Superoxide dismutase, Antioxidant, Glutathione reductase, Oxidative stress, Chemistry, Glutathione, Pharmacology, Cardiac marker, Biochemistry, Internal medicine, Enzyme, Endocrinology, Troponin, Medicine, Myocardial infarction, Pathology
- MeSH
- animals, humans, rats, withania, plant leaves, myocardial infarction, isoproterenol, glutathione peroxidase, superoxide dismutase, lipids, troponin i, plant extracts, antioxidants, lipid peroxidation, oxidative stress, biomarkers
8 authors
From MY, BD
- Md. Ibrahim KhalilUniversiti Sains Malaysia; Jahangirnagar University
- Istiyak AhmmedJahangirnagar University
- Romana AhmedJahangirnagar University
- E. M. TanvirJahangirnagar University
- Rizwana AfrozJahangirnagar University
- Sudip PaulJahangirnagar University
Abstract
We investigated the protective role of Withania somnifera leaf extract (WSLEt) on isoproterenol- (ISO-) induced myocardial infarction (MI) in rats. Subcutaneous injection of ISO (85 mg/kg body weight (b.w.)) administered to rats for two consecutive days caused a significant increase in cardiac troponin I (cTnI) levels and serum lipid profiles, as well as the activities of some marker enzymes. In addition to these diagnostic markers, there were increased levels of lipid peroxidation (LPO) and decreased activities of enzymatic antioxidants (superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GRx), and glutathione-S-transferase (GST)) in the myocardium. However, oral pretreatment (100 mg/kg b.w.) with WSLEt for 4 weeks elicited a significant cardioprotective activity by lowering the levels of cTnI, lipid profiles, and marker enzymes. The levels of LPO products were also significantly decreased. Elevated activities of antioxidant enzymes were also observed in rats pretreated with WSLEt. As further confirmed histopathologically, our findings strongly suggest that the cardioprotective effect of WSLEt on myocardium experiencing ISO-induced oxidative damage may be due to an augmentation of the endogenous antioxidant system and an inhibition of LPO in the myocardial membrane. We conclude that WSLEt confers some protection against oxidative damage in ISO-induced MI in rats.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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