Blueberry Component Pterostilbene Protects Corneal Epithelial Cells from Inflammation via Anti-oxidative Pathway
Li J, Ruzhi Deng, Hua X, Zhang L, Lu F, Coursey TG, Pflugfelder SC, Li DQ
Scientific reports · 86 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Nonprofit
- Research to Prevent Blindness
- Government
- National Institutes of Health
- Government
- National Eye Institute
- Government
- NEI NIH HHS
- Grants
- National Eye Institute (R01 EY011915); National Eye Institute (P30 EY002520); National Eye Institute (R01EY023598); Research to Prevent Blindness (EY002520); National Eye Institute (EY002520); National Eye Institute (EY011915); National Eye Institute (EY023598); National Institutes of Health (R01 EY023598); National Institutes of Health (EY023598); National Institutes of Health (EY011915); National Institutes of Health (EY002520)
Based on 4 listed funder(s).
Publication
- Published
- 2016-01-14 · Sci Rep · vol. 6 · issue 1 · p. 19408
- Publisher
- Nature Portfolio
- Cited
- 117 citations · more than 99% of similar papers · 11.1× the field average
- Impact
- Top 10% most cited in its field
- References
- 54 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Corneal Surgery and Treatments · Ocular Surface and Contact Lens · Glaucoma and retinal disorders
- Keywords
- Pterostilbene, Oxidative stress, Reactive oxygen species, Inflammation, Superoxide dismutase, Chemistry, Proinflammatory cytokine, Antioxidant, Malondialdehyde, Corneal epithelium, Biochemistry, Pharmacology, Biology, Immunology, Cornea, Resveratrol
- MeSH
- epithelium, corneal, epithelial cells, humans, keratitis, reactive oxygen species, stilbenes, inflammation mediators, cytokines, antioxidants, signal transduction, gene expression, gene expression regulation, enzymologic, oxidation-reduction, oxidative stress, osmolar concentration, adult, aged, middle aged, young adult, blueberry plants
8 authors
From US, CN
- Jin LiBaylor College of Medicine; Wenzhou Medical University; Affiliated Eye Hospital of Wenzhou Medical College; Ocular Surface Center
- Ruzhi DengBaylor College of Medicine; Wenzhou Medical University; Affiliated Eye Hospital of Wenzhou Medical College; Ocular Surface Center
- Xia HuaBaylor College of Medicine; Ocular Surface Center; Tianjin Medical University Eye Hospital; Tianjin Medical University
- Lili ZhangBaylor College of Medicine; Ocular Surface Center
- Fan LüWenzhou Medical University; Affiliated Eye Hospital of Wenzhou Medical College
- Terry G. CourseyBaylor College of Medicine; Ocular Surface Center
Abstract
Blueberries have been recognized to possess protective properties from inflammation and various diseases, but not for eye and ocular disorders. This study explores potential benefits of pterostilbene (PS), a natural component of blueberries, in preventing ocular surface inflammation using an in vitro culture model of human corneal epithelial cells (HCECs) exposed to hyperosmotic medium at 450 mOsM. Gene expression was detected by RT-qPCR, and protein production or activity was determined by ELISA, zymography, Western blotting and immunofluorescent staining. Reactive oxygen species (ROS) production was measured using DCFDA kit. The addition of PS significantly reduced the expression of pro-inflammatory mediators, TNF-α, IL-1 β, IL-6, MMP-2 and MMP-9 in HCECs exposed to hyperosmotic medium. Pre-treatment with PS (5 to 20 μM) suppressed ROS overproduction in a dose-dependent manner. Additionally, PS significantly decreased the levels of oxidative damage biomarkers, malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), aconitase-2 and 8-hydroxydeoxyguanosine (8-OHdG). Importantly, PS was found to rebalance homeostasis between oxygenases and anti-oxidative enzymes by decreasing cyclooxygenase 2 (COX2) expression and restoring the activity of antioxidant enzymes, superoxide dismutase 1 (SOD1) and peroxiredoxin-4 (PRDX4) during hyperosmotic stress. Our findings demonstrate that PS protects human cornea from hyperosmolarity-induced inflammation and oxidative stress, suggesting protective effects of PS on dry eye.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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