D, L-Sulforaphane Loaded Fe3O4@ Gold Core Shell Nanoparticles: A Potential Sulforaphane Delivery System
Kheiri Manjili H, Ma'mani L, Tavaddod S, Mashhadikhan M, Shafiee A, Naderi-Manesh H
PloS one · 34 citations
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How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
Based on full-text disclosure statement.
Publication
- Published
- 2016-03-16 · PLoS One · vol. 11 · issue 3 · p. e0151344
- Publisher
- Public Library of Science
- Cited
- 62 citations · more than 92% of similar papers · 3.2× the field average
- Impact
- Top 10% most cited in its field
- References
- 39 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Genomics, phytochemicals, and oxidative stress · Synthesis and Characterization of Heterocyclic Compounds · Adenosine and Purinergic Signaling
- Keywords
- Sulforaphane, Polyethylene glycol, Nanoparticle, Nuclear chemistry, HeLa, Chemistry, Colloidal gold, Apoptosis, Cytotoxicity, Materials science, Nanotechnology, Biochemistry, In vitro
- MeSH
- humans, gold, ferric compounds, isothiocyanates, sulfoxides, microscopy, electron, drug delivery systems, spectroscopy, fourier transform infrared, spectrophotometry, ultraviolet, spectrometry, x-ray emission, apoptosis, metal nanoparticles, mcf-7 cells
6 authors
From IR
- Hamidreza Kheiri ManjiliTarbiat Modares University
- Leila Ma’maniAgricultural Research & Education Organization; Agricultural Biotechnology Research Institute of Iran
- Sharareh TavaddodTarbiat Modares University
- Maedeh MashhadikhanIslamic Azad University, Science and Research Branch
- Abbas Shafiee · correspondingTehran University of Medical Sciences
- Hossein Naderi‐Manesh · correspondingTarbiat Modares University
Abstract
A novel design of gold-coated iron oxide nanoparticles was fabricated as a potential delivery system to improve the efficiency and stability of d, l-sulforaphane as an anticancer drug. To this purpose, the surface of gold-coated iron oxide nanoparticles was modified for sulforaphane delivery via furnishing its surface with thiolated polyethylene glycol-folic acid and thiolated polyethylene glycol-FITC. The synthesized nanoparticles were characterized by different techniques such as FTIR, energy dispersive X-ray spectroscopy, UV-visible spectroscopy, scanning and transmission electron microscopy. The average diameters of the synthesized nanoparticles before and after sulforaphane loading were obtained ∼ 33 nm and ∼ 38 nm, respectively, when ∼ 2.8 mmol/g of sulforaphane was loaded. The result of cell viability assay which was confirmed by apoptosis assay on the human breast cancer cells (MCF-7 line) as a model of in vitro-cancerous cells, proved that the bare nanoparticles showed little inherent cytotoxicity, whereas the sulforaphane-loaded nanoparticles were cytotoxic. The expression rate of the anti-apoptotic genes (bcl-2 and bcl-xL), and the pro-apoptotic genes (bax and bak) were quantified, and it was found that the expression rate of bcl-2 and bcl-xL genes significantly were decreased when MCF-7 cells were incubated by sulforaphane-loaded nanoparticles. The sulforaphane-loaded into the designed gold-coated iron oxide nanoparticles, acceptably induced apoptosis in MCF-7 cells.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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