Funded in part by Battelle
Free 25-Hydroxyvitamin D: Impact of Vitamin D Binding Protein Assays on Racial-Genotypic Associations
Nielson CM, Jones KS, Chun RF, Jacobs JM, Wang Y, Hewison M, Adams JS, Swanson CM, Lee CG, Vanderschueren D, Pauwels S, Prentice A, Smith RD, Shi T, Gao Y, Schepmoes AA, Zmuda JM, Lapidus J, Cauley JA, Bouillon R, Schoenmakers I, Orwoll ES, Osteoporotic Fractures in Men (MrOS) Research Group
The Journal of clinical endocrinology and metabolism · 134 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Cross-sectional study (indexed by PubMed)
- Studied in
- People
- Main outcome
- Health markers and function
Who paid for it
- Funding
- Industry funded
- Government
- U.S. Department of Energy
- Company
- Battelle
- Government
- Department for International Development
- Government
- Fonds Wetenschappelijk Onderzoek
- University or hospital
- KU Leuven
- Government
- Vlaamse regering
- Government
- National Institutes of Health
- Government
- Medical Research Council
- Government
- National Institute on Aging
- Government
- National Institute of General Medical Sciences
- Government
- National Institute of Diabetes and Digestive and Kidney Diseases
- Government
- National Institute of Arthritis and Musculoskeletal and Skin Diseases
- Government
- National Center for Advancing Translational Sciences
- Government
- Pacific Northwest National Laboratory
- Government
- NIAMS NIH HHS
- Government
- NIA NIH HHS
- Government
- NIGMS NIH HHS
- Government
- NIDDK NIH HHS
- Government
- NCATS NIH HHS
- Grants
- National Center for Advancing Translational Sciences (UL-1TR000124); U.S. Department of Energy (DE-AC05–76RL01830); Pacific Northwest National Laboratory (DEAC0576RL01830); National Institute on Aging (U01AG042168); National Institute of Arthritis and Musculoskeletal and Skin Diseases (U01 AG042145); Medical Research Council (MC-A760–5QX00); National Institute of Arthritis and Musculoskeletal and Skin Diseases (U01 AG042140); Department for International Development (MCA760‐5QX00); National Center for Advancing Translational Sciences (UL1TR000128); National Institute on Aging (AG042140); National Center for Advancing Translational Sciences (U01 AG042124); U.S. Department of Energy (DE-AC05?); National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR063910); National Center for Advancing Translational Sciences (U01 AG042168); National Institute of Arthritis and Musculoskeletal and Skin Diseases (U01 AG042143); National Institute on Aging (AG042139); National Center for Advancing Translational Sciences (U01AG042145); National Institute of Diabetes and Digestive and Kidney Diseases (T32 DK007674); Battelle (DE- AC05-76RL01830); Medical Research Council (U105960371); National Institute of Arthritis and Musculoskeletal and Skin Diseases (U01 AG027810); National Institute of Arthritis and Musculoskeletal and Skin Diseases (UL1 TR000128); Fonds Wetenschappelijk Onderzoek (G. 0858.11); Department for International Development (U105960371); National Institute on Aging (U01 AG042124); National Institute of Arthritis and Musculoskeletal and Skin Diseases (P50AR063020); National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR063910); National Center for Advancing Translational Sciences (U01 AG042139); National Institute on Aging (AG042124); National Institute of Arthritis and Musculoskeletal and Skin Diseases (K01 AR062655); U.S. Department of Energy (DE-AC05-76RL0); National Center for Advancing Translational Sciences (U01 AG-042140); National Institute of General Medical Sciences (P41-GM103493); Battelle (DE-AC05); National Center for Advancing Translational Sciences (U01 AR066160); National Institute of Arthritis and Musculoskeletal and Skin Diseases (U01 AR066160); KU Leuven (GOA 15/0/01); National Center for Advancing Translational Sciences (U01 AG042143); National Institute on Aging (U01AG042139); National Institute on Aging (U01 AG-042140); National Institute on Aging (U01 AG027810); National Institute of Arthritis and Musculoskeletal and Skin Diseases (U01 AG042139); National Center for Advancing Translational Sciences (TR000128); Department for International Development (U123261351); National Institute on Aging (AG027810); Medical Research Council (U123261351); National Institute of Arthritis and Musculoskeletal and Skin Diseases (U01 AG042124); National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR066160); National Center for Advancing Translational Sciences (U01 AG027810); National Institute on Aging (AG042143); National Institute on Aging (U01AG042145); National Institute on Aging (U01 AG042143); National Institutes of Health (AG042168); National Institutes of Health (AG042139); National Institutes of Health (U01 AG042124); National Institutes of Health (AR066160); National Institutes of Health (UL1TR000128); National Institutes of Health (U01 AG042168); National Institutes of Health (AG042140); National Institutes of Health (U01-AG027810); National Institutes of Health (AR063910); National Institutes of Health (U01 AG042139); National Institutes of Health (U01 AG042145); National Institutes of Health (AG027810); National Institutes of Health (P41-GM103493); National Institutes of Health (TR000128); National Institutes of Health (U01AR066160); National Institutes of Health (U01 AG042143); National Institutes of Health (AG042124); National Institutes of Health (K01AR062655); National Institutes of Health (T32 DK007674-20); National Institutes of Health (R01 AR063910); National Institutes of Health (AG042143); National Institutes of Health (U01 AG042140); National Institutes of Health (AG042145); National Institute of Arthritis and Musculoskeletal and Skin Diseases (U01 AG042168); National Institutes of Health (DE-AC05-76RL01830)
Based on 19 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2016-03-23 · J Clin Endocrinol Metab · vol. 101 · issue 5 · pp. 2226–2234
- Publisher
- Oxford University Press
- Cited
- 165 citations · more than 100% of similar papers · 22.8× the field average
- Impact
- Top 10% most cited in its field
- References
- 40 works
- Access
- Open access (hybrid journal) · CC-BY-NC
- Research areas
- Vitamin D Research Studies · Bone health and osteoporosis research · HIV-related health complications and treatments
- Keywords
- Vitamin D-binding protein, Vitamin D and neurology, Polyclonal antibodies, Monoclonal, Medicine, Internal medicine, vitamin D deficiency, Genotype, Monoclonal antibody, Endocrinology, Demography, Immunology, Biology, Genetics, Antibody, Gene, Sociology
- MeSH
- humans, vitamin d, vitamin d-binding protein, cross-sectional studies, adult, aged, male, white people, black or african american, black people
22 authors
From US, GB, BE, GM
- Carrie M. NielsonOregon Health & Science University
- Kerry S. JonesOregon Health & Science University; MRC Elsie Widdowson Laboratory; Medical Research Council
- Rene F. ChunUniversity of California, Los Angeles; Oregon Health & Science University
- Jon JacobsPacific Northwest National Laboratory; Oregon Health & Science University
- Ying WangOregon Health & Science University
- Martin HewisonOregon Health & Science University; University of Birmingham
Abstract
Context
Total 25-hydroxyvitamin D (25OHD) is a marker of vitamin D status and is lower in African Americans than in whites. Whether this difference holds for free 25OHOD (f25OHD) is unclear, considering reported genetic-racial differences in vitamin D binding protein (DBP) used to calculate f25OHD.
Objectives
Our objective was to assess racial-geographic differences in f25OHD and to understand inconsistencies in racial associations with DBP and calculated f25OHD.
Design
This study used a cross-sectional design.
Setting
The general community in the United States, United Kingdom, and The Gambia were included in this study.
Participants
Men in Osteoporotic Fractures in Men and Medical Research Council studies (N = 1057) were included.
Exposures
Total 25OHD concentration, race, and DBP (GC) genotype exposures were included.
Outcome measures
Directly measured f25OHD, DBP assessed by proteomics, monoclonal and polyclonal immunoassays, and calculated f25OHD were the outcome measures.
Results
Total 25OHD correlated strongly with directly measured f25OHD (Spearman r = 0.84). Measured by monoclonal assay, mean DBP in African-ancestry subjects was approximately 50% lower than in whites, whereas DBP measured by polyclonal DBP antibodies or proteomic methods was not lower in African-ancestry. Calculated f25OHD (using polyclonal DBP assays) correlated strongly with directly measured f25OHD (r = 0.80-0.83). Free 25OHD, measured or calculated from polyclonal DBP assays, reflected total 25OHD concentration irrespective of race and was lower in African Americans than in US whites.
Conclusions
Previously reported racial differences in DBP concentration are likely from monoclonal assay bias, as there was no racial difference in DBP concentration by other methods. This confirms the poor vitamin D status of many African-Americans and the utility of total 25OHD in assessing vitamin D in the general population.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY-NC).
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