Eicosapentaenoic acid and arachidonic acid differentially regulate adipogenesis, acquisition of a brite phenotype and mitochondrial function in primary human adipocytes
Fleckenstein-Elsen M, Dinnies D, Jelenik T, Roden M, Romacho T, Eckel J
Molecular nutrition & food research · 63 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Funding not disclosed
Publication
- Published
- 2016-05-09 · Mol Nutr Food Res · vol. 60 · issue 9 · pp. 2065–2075
- Publisher
- Wiley
- Cited
- 70 citations · more than 97% of similar papers · 6.0× the field average
- Impact
- Top 10% most cited in its field
- References
- 59 works
- Access
- Open access (repository copy) · OTHER-OA
- Research areas
- Adipose Tissue and Metabolism · Adipokines, Inflammation, and Metabolic Diseases · Cardiovascular Disease and Adiposity
- Keywords
- Adipogenesis, Eicosapentaenoic acid, Biology, Polyunsaturated fatty acid, Adipocyte, Arachidonic acid, Endocrinology, Mitochondrion, Docosahexaenoic acid, Citrate synthase, Mitochondrial respiratory chain, Adipose tissue, Internal medicine, Fatty acid, Biochemistry, Medicine
- MeSH
- cells, cultured, mitochondria, adipocytes, humans, citrate (si)-synthase, carnitine o-palmitoyltransferase, docosahexaenoic acids, eicosapentaenoic acid, arachidonic acid, cell differentiation, female, adipogenesis, uncoupling protein 1
6 authors
From DE
- Manuela Fleckenstein‐ElsenDeutsches Diabetes-Zentrum e.V.; German Center for Diabetes Research
- Daniela DinniesDeutsches Diabetes-Zentrum e.V.; German Center for Diabetes Research
- Tomáš JeleníkDeutsches Diabetes-Zentrum e.V.; German Center for Diabetes Research
- Michael RodenDeutsches Diabetes-Zentrum e.V.; German Center for Diabetes Research; Heinrich Heine University Düsseldorf
- Tania RomachoDeutsches Diabetes-Zentrum e.V.; German Center for Diabetes Research
- Jürgen Eckel · correspondingDeutsches Diabetes-Zentrum e.V.; German Center for Diabetes Research
Abstract
Scope
n-3 and n-6 PUFAs have several opposing biological effects and influence white adipose tissue (WAT) function. The recent discovery of thermogenic UCP1-expressing brite adipocytes within WAT raised the question whether n-3 and n-6 PUFAs exert differential effects on brite adipocyte formation and mitochondrial function.
Methods and results
Primary human preadipocytes were treated with n-3 PUFAs (eicosapentaenoic acid, EPA; docosahexaenoic acid, DHA) or n-6 PUFA (arachidonic acid, ARA) during differentiation, and adipogenesis, white and brite gene expression markers, mitochondrial content and function were analyzed at day 12 of differentiation. Adipogenesis was equally increased by n-3 and n-6 PUFAs. The n-6 PUFA ARA increased lipid droplet size and expression of the white-specific marker TCF21 while decreased mitochondrial protein expression and respiratory function. In contrast, EPA increased expression of the brown adipocyte-related genes UCP1 and CPT1B, and improved mitochondrial function of adipocytes. The opposing effects of EPA and ARA on gene expression and mitochondrial function were also observed in cells treated from day 8 to 12 of adipocyte differentiation.
Conclusion
EPA promotes brite adipogenesis and improves parameters of mitochondrial function, such as increased expression of CPTB1, citrate synthase activity and higher maximal respiratory capacity, while ARA reduced mitochondrial spare respiratory capacity in vitro.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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