Withania somnifera Induces Cytotoxic and Cytostatic Effects on Human T Leukemia Cells
Turrini E, Calcabrini C, Sestili P, Catanzaro E, de Gianni E, Diaz AR, Hrelia P, Tacchini M, Guerrini A, Canonico B, Papa S, Valdrè G, Fimognari C
Toxins · 24 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
Based on full-text disclosure statement.
Publication
- Published
- 2016-05-12 · Toxins (Basel) · vol. 8 · issue 5 · p. 147
- Publisher
- Multidisciplinary Digital Publishing Institute
- Cited
- 44 citations · more than 96% of similar papers · 8.0× the field average
- Impact
- Top 10% most cited in its field
- References
- 60 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Phytochemicals and Medicinal Plants · Medicinal Plants and Neuroprotection · Andrographolide Research and Applications
- Keywords
- Withania somnifera, Cytotoxic T cell, Pharmacology, Genotoxicity, Leukemia, Biology, Cancer, Traditional medicine, Immunology, Cancer research, Toxicity, Medicine, In vitro, Biochemistry, Pathology, Internal medicine
- MeSH
- jurkat cells, humans, withania, plant roots, leukemia, t-cell, dna damage, calcium, calreticulin, adenosine triphosphate, plant extracts, antineoplastic agents, mutagens, apoptosis, oxidative stress, hsp70 heat-shock proteins, hsp90 heat-shock proteins
13 authors
From IT
- Eleonora TurriniUniversity of Bologna
- Cinzia CalcabriniUniversity of Bologna
- Piero SestiliUniversity of Urbino
- Elena CatanzaroUniversity of Bologna
- Elena De GianniUniversity of Bologna
- Anna DiazUniversity of Urbino
Abstract
Cancer chemotherapy is characterized by an elevated intrinsic toxicity and the development of drug resistance. Thus, there is a compelling need for new intervention strategies with an improved therapeutic profile. Immunogenic cell death (ICD) represents an innovative anticancer strategy where dying cancer cells release damage-associated molecular patterns promoting tumor-specific immune responses. The roots of Withania somnifera (W. somnifera) are used in the Indian traditional medicine for their anti-inflammatory, immunomodulating, neuroprotective, and anticancer activities. The present study is designed to explore the antileukemic activity of the dimethyl sulfoxide extract obtained from the roots of W. somnifera (WE). We studied its cytostatic and cytotoxic activity, its ability to induce ICD, and its genotoxic potential on a human T-lymphoblastoid cell line by using different flow cytometric assays. Our results show that WE has a significant cytotoxic and cytostatic potential, and induces ICD. Its proapoptotic mechanism involves intracellular Ca(2+) accumulation and the generation of reactive oxygen species. In our experimental conditions, the extract possesses a genotoxic potential. Since the use of Withania is suggested in different contexts including anti-infertility and osteoarthritis care, its genotoxicity should be carefully considered for an accurate assessment of its risk-benefit profile.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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