Post-exercise cold water immersion does not alter high intensity interval training-induced exercise performance and Hsp72 responses, but enhances mitochondrial markers
Aguiar PF, Magalhães SM, Fonseca IA, da Costa Santos VB, de Matos MA, Peixoto MF, Nakamura FY, Crandall C, Araújo HN, Silveira LR, Rocha-Vieira E, de Castro Magalhães F, Amorim FT
Cell stress & chaperones · 19 citations
How it was studied
- Design
- Controlled clinical trial (classified by our AI screen)
- Studied in
- People
- Main outcome
- Health markers and function
Who paid for it
- Funding
- Independent funding
- Government
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Government
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Government
- Fundação de Amparo à Pesquisa do Estado de Minas Gerais
- Government
- CNPQ
- Government
- FAPEMIG
- Government
- CAPES
- Grants
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (2014-4); Conselho Nacional de Desenvolvimento Científico e Tecnológico (445096/2014-4); Fundação de Amparo à Pesquisa do Estado de Minas Gerais (APQ-01382-12); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (PNPD-2455/2011); Conselho Nacional de Desenvolvimento Científico e Tecnológico (2013-4); Conselho Nacional de Desenvolvimento Científico e Tecnológico (2014-4); Conselho Nacional de Desenvolvimento Científico e Tecnológico (407252/2013-4)
Based on 6 listed funder(s).
Publication
- Published
- 2016-06-08 · Cell Stress Chaperones · vol. 21 · issue 5 · pp. 793–804
- Publisher
- Springer Science+Business Media
- Cited
- 27 citations · more than 90% of similar papers · 2.9× the field average
- Impact
- Top 10% most cited in its field
- References
- 51 works
- Access
- Open access (hybrid journal) · CC-BY-NC-ND
- Research areas
- Exercise and Physiological Responses · Cardiovascular and exercise physiology · Adipose Tissue and Metabolism
- Keywords
- High-intensity interval training, TFAM, Citrate synthase, Mitochondrial biogenesis, Internal medicine, Endocrinology, Interval training, NRF1, AMPK, Protein kinase A, Medicine, Biology, Kinase, Chemistry, Mitochondrion, Biochemistry
- MeSH
- muscle, skeletal, mitochondria, muscle, humans, mitochondrial proteins, adaptation, physiological, adult, male, hsp72 heat-shock proteins, cold temperature, young adult, physical conditioning, human, biomarkers, high-intensity interval training
13 authors
From BR, US
- Paula Fernandes de AguiarUniversidade Federal dos Vales do Jequitinhonha e Mucuri
- Sílvia Mourão MagalhãesUniversidade Federal dos Vales do Jequitinhonha e Mucuri
- Ivana Alice ixeira Te FonsecaUniversidade Federal dos Vales do Jequitinhonha e Mucuri
- Vanessa Batista da Costa SantosUniversidade Estadual de Londrina
- Mariana Aguiar de MatosUniversidade Federal dos Vales do Jequitinhonha e Mucuri
- Marco Fabrício Dias‐PeixotoUniversidade Federal dos Vales do Jequitinhonha e Mucuri
Abstract
This study aims to evaluate the effect of regular post-exercise cold water immersion (CWI) on intramuscular markers of cellular stress response and signaling molecules related to mitochondria biogenesis and exercise performance after 4 weeks of high intensity interval training (HIIT). Seventeen healthy subjects were allocated into two groups: control (CON, n = 9) or CWI (n = 8). Each HIIT session consisted of 8-12 cycling exercise stimuli (90-110 % of peak power) for 60 s followed by 75 s of active recovery three times per week, for 4 weeks (12 HIIT sessions). After each HIIT session, the CWI had their lower limbs immersed in cold water (10 °C) for 15 min and the CON recovered at room temperature. Exercise performance was evaluated before and after HIIT by a 15-km cycling time trial. Vastus lateralis biopsies were obtained pre and 72 h post training. Samples were analyzed for heat shock protein 72 kDa (Hsp72), adenosine monophosphate-activated protein kinase (AMPK), and phosphorylated p38 mitogen-activated protein kinase (p-p38 MAPK) assessed by western blot. In addition, the mRNA expression of heat shock factor-1 (HSF-1), peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α), nuclear respiratory factor 1 and 2 (NRF1 and 2), mitochondrial transcription factor A (Tfam), calcium calmodulin-dependent protein kinase 2 (CaMK2) and enzymes citrate synthase (CS), carnitine palmitoyltransferase I (CPT1), and pyruvate dehydrogenase kinase (PDK4) were assessed by real-time PCR. Time to complete the 15-km cycling time trial was reduced with training (p 0.05). No differences were observed with training or condition for mRNA expression of PGC-1α (p = 0.31), CPT1 (p = 0.14), CS (p = 0.44), and NRF-2 (p = 0.82). However, HFS-1 (p = 0.007), PDK4 (p = 0.03), and Tfam (p = 0.03) mRNA were higher in CWI. NRF-1 decrease in both groups after training (p = 0.006). CaMK2 decreased with HIIT (p = 0.003) but it was not affected by CWI (p = 0.99). Cold water immersion does not alter HIIT-induced Hsp72, AMPK, p38 MAPK, and exercise performance but was able to increase some markers of cellular stress response and signaling molecules related to mitochondria biogenesis.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY-NC-ND).
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