Cell lineage responses to photobiomodulation therapy
Engel KW, Khan I, Arany PR
Journal of biophotonics · 50 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- National Institutes of Health
- Government
- National Institute of Dental and Craniofacial Research
- Government
- Intramural Research program of the National Institute of Dental and Craniofacial Research
Based on 3 listed funder(s).
Publication
- Published
- 2016-07-08 · J Biophotonics · vol. 9 · issue 11-12 · pp. 1148–1156
- Publisher
- Wiley
- Cited
- 69 citations · more than 97% of similar papers · 5.9× the field average
- Impact
- Top 10% most cited in its field
- References
- 37 works
- Access
- Paywalled
- Research areas
- Laser Applications in Dentistry and Medicine · Bee Products Chemical Analysis · Dermatologic Treatments and Research
- Keywords
- Fibroblast, Catalase, Low level laser therapy, Keratinocyte, Laser therapy, In vivo, Cell, Medicine, Cell growth, Cell therapy, Chemistry, In vitro, Cancer research, Biology, Internal medicine, Laser, Oxidative stress, Biochemistry
- MeSH
- cells, cultured, fibroblasts, keratinocytes, humans, reactive oxygen species, dose-response relationship, radiation, wound healing, cell lineage, low-level light therapy
3 authors
From US
- Karl W. EngelNational Institute of Dental and Craniofacial Research
- Imran Ahmad KhanNational Institute of Dental and Craniofacial Research
- Praveen Ravindra Arany · correspondingNational Institute of Dental and Craniofacial Research; University at Buffalo, State University of New York
Abstract
Photobiomodulation (PBM) therapy has been noted to promote cell proliferation and growth in many different cell types shown both in vitro and in vivo. Currently, treatment regimens are used in the clinic for a variety of ailments, including wound healing. However, most protocols treat an anatomical site without considering individual cell types constituting the target tissues. This study investigates the maximal dose threshold for oral keratinocyte and fibroblast cell types treated with near-infrared laser therapy. We observed keratinocytes have increased sensitivity to laser irradiances (>0.047 W/cm2 , 300 sec, 14.2 J/cm2 ) compared to the fibroblast cells (>0.057 W/cm2 , 300 sec, 15.1 J/cm2 ) (p < 0.0001). Laser treatments were noted to generate increased reactive oxygen species (ROS) levels in keratinocytes compared to fibroblasts that appeared to inversely correlate with higher basal catalase expression. To validate these observations, melatonin was used to treat keratinocytes to induce catalase activity (p < 0.0001). Increased melatonin-induced catalase levels were noted to significantly improve keratinocyte survival to phototoxic laser doses. These observations suggest that clinical laser dosing should account for differential effects of lasers on individual cell types to improve safety and clinical efficacy of PBM therapy.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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