Differential Responses to Vitamin D2 and Vitamin D3 Are Associated With Variations in Free 25-Hydroxyvitamin D
Chun RF, Hernandez I, Pereira R, Swinkles L, Huijs T, Zhou R, Liu NQ, Shieh A, Guemes M, Mallya SM, Adams JS, Hewison M
Endocrinology · 31 citations
How it was studied
- Design
- Animal study (classified by our AI screen)
- Studied in
- Animals
- Main outcome
- Health markers and function
Who paid for it
- Funding
- Independent funding
- Government
- National Institute of Arthritis and Musculoskeletal and Skin Diseases
- Government
- NIAMS NIH HHS
- Grants
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (P50AR063020); National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR063910)
Based on 2 listed funder(s).
Publication
- Published
- 2016-07-11 · Endocrinology · vol. 157 · issue 9 · pp. 3420–3430
- Publisher
- Oxford University Press
- Cited
- 48 citations · more than 95% of similar papers · 4.6× the field average
- Impact
- Top 10% most cited in its field
- References
- 45 works
- Access
- Free to read
- Research areas
- Vitamin D Research Studies · Bone health and osteoporosis research · Adrenal Hormones and Disorders
- Keywords
- Internal medicine, Endocrinology, Vitamin D and neurology, Vitamin, Osteoclast, Osteoblast, Calcitriol, Apposition, Ergocalciferol, Chemistry, Cholecalciferol, Bone histomorphometry, Medicine, Osteoporosis, In vitro, Receptor, Trabecular bone
- MeSH
- bone and bones, kidney, spleen, cell line, animals, mice, inbred c57bl, humans, cholecalciferol, ergocalciferols, vitamin d, female, male
12 authors
From US, GB, NL
- Rene F. ChunUniversity of California, Los Angeles
- Alejandro Ivan HernandezUniversity of Birmingham
- Renata C. PereiraUniversity of California, Los Angeles
- Leon SwinklesFuture Diagnostics (Netherlands)
- Tonnie HuijsFuture Diagnostics (Netherlands)
- Rui ZhouUniversity of California, Los Angeles
Abstract
25-Hydroxyvitamin D (25D) circulates bound primarily to serum vitamin D binding protein (DBP), with DBP showing higher binding affinity for 25D3 than 25D2. We therefore hypothesized that vitamin D2 (D2) promotes higher serum levels of unbound 25D (free 25D), with different functional responses, relative to vitamin D3 (D3). Week 3 C56BL/6 mice were placed on diets containing either D2 or D3 alone (both 1000 IU/kg). At week 8 and week 16, D2 mice had only 25D2 in circulation (26.6 ± 1.9 and 33.3 ± 4.4 ng/mL), and D3 mice had only 25D3 (28.3 ± 2.0 and 31.7 ± 2.1 ng/mL). At week 8 (44.5 ± 6.4 vs 62.4 ± 11.6 pg/mL, P < .05) and week 16 (78.4 ± 12.6 vs 95.5 ± 11.6), D2 mice had lower serum 1,25-dihydroxyvitamin D relative to D3 mice. By contrast, measured free 25D was significantly higher in D2 mice at week 8 (16.8 ± 0.65 vs 8.4 ± 0.63 pg/mL, P < .001) and week 16 (17.4 ± 0.43 vs 8.4 ± 0.44, P < .001). A two-way ANOVA of bone histomorphometry showed that week 8 D2 mice had significantly higher osteoclast surface/bone surface, eroded surface/bone surface, and mineral apposition rate compared with D3 mice. Osteoblast surface/bone surface was higher in week 8 D2 females but not week 8 D2 males. At week 16, D2 mice had significantly higher bone volume/total volume and trabecular number compared with D3 mice. Differences in bone phenotype were observed despite D2 mice reaching similar serum 25D levels and lower 1,25D levels compared with D3 mice. These data indicate that 25D2 binds less well to DBP than 25D3, with resulting higher levels of free 25D promoting differential effects on bone in mice exposed to D2 alone.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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