Funded in part by Meat and Livestock Australia
Divergent shifts in lipid mediator profile following supplementation with n-3 docosapentaenoic acid and eicosapentaenoic acid
Markworth JF, Kaur G, Miller EG, Larsen AE, Sinclair AJ, Maddipati KR, Cameron-Smith D
FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 75 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Randomized controlled trial (classified by our AI screen)
- Studied in
- People
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Industry funded
- Industry group
- Meat and Livestock Australia
- Government
- National Institutes of Health
- Government
- National Center for Research Resources
- Government
- NCRR NIH HHS
- Grants
- National Center for Research Resources (S10-RR-027926)
Based on 4 listed funder(s).
Publication
- Published
- 2016-07-26 · FASEB J · vol. 30 · issue 11 · pp. 3714–3725
- Publisher
- Wiley
- Cited
- 88 citations · more than 99% of similar papers · 10.7× the field average
- Impact
- Top 10% most cited in its field
- References
- 75 works
- Access
- Open access (repository copy)
- Research areas
- Fatty Acid Research and Health · Eicosanoids and Hypertension Pharmacology · Lipid metabolism and biosynthesis
- Keywords
- Docosapentaenoic acid, Eicosapentaenoic acid, Docosahexaenoic acid, Polyunsaturated fatty acid, Chemistry, Lipid signaling, Biochemistry, Fatty acid, Lipidomics, Food science, Enzyme
- MeSH
- humans, fatty acids, omega-3, eicosapentaenoic acid, fatty acids, fatty acids, unsaturated, diet, cross-over studies, dietary supplements, adult, female, lipid metabolism, young adult
7 authors
From NZ, AU, US
- James F. Markworth · correspondingUniversity of Auckland
- Gunveen KaurDeakin University
- Eliza G. MillerDeakin University
- Amy LarsenLa Trobe University
- Andrew James SinclairDeakin University
- Krishna Rao MaddipatiWayne State University; The Barbara Ann Karmanos Cancer Institute
Abstract
In contrast to the well-characterized effects of specialized proresolving lipid mediators (SPMs) derived from eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), little is known about the metabolic fate of the intermediary long-chain (LC) n-3 polyunsaturated fatty acid (PUFA) docosapentaenoic acid (DPA). In this double blind crossover study, shifts in circulating levels of n-3 and n-6 PUFA-derived bioactive lipid mediators were quantified by an unbiased liquid chromatography-tandem mass spectrometry lipidomic approach. Plasma was obtained from human subjects before and after 7 d of supplementation with pure n-3 DPA, n-3 EPA or placebo (olive oil). DPA supplementation increased the SPM resolvin D5n-3DPA (RvD5n-3DPA) and maresin (MaR)-1, the DHA vicinal diol 19,20-dihydroxy-DPA and n-6 PUFA derived 15-keto-PG E2 (15-keto-PGE2). EPA supplementation had no effect on any plasma DPA or DHA derived mediators, but markedly elevated monohydroxy-eicosapentaenoic acids (HEPEs), including the e-series resolvin (RvE) precursor 18-HEPE; effects not observed with DPA supplementation. These data show that dietary n-3 DPA and EPA have highly divergent effects on human lipid mediator profile, with no overlap in PUFA metabolites formed. The recently uncovered biologic activity of n-3 DPA docosanoids and their marked modulation by dietary DPA intake reveals a unique and specific role of n-3 DPA in human physiology.-Markworth, J. F., Kaur, G., Miller, E. G., Larsen, A. E., Sinclair, A. J., Maddipati, K. R., Cameron-Smith, D. Divergent shifts in lipid mediator profile following supplementation with n-3 docosapentaenoic acid and eicosapentaenoic acid.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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