Harnessing the Power of Cruciferous Vegetables: Developing a Biomarker for Brassica Vegetable Consumption Using Urinary 3,3'-Diindolylmethane
Fujioka N, Ransom BW, Carmella SG, Upadhyaya P, Lindgren BR, Roper-Batker A, Hatsukami DK, Fritz VA, Rohwer C, Hecht SS
Cancer prevention research (Philadelphia, Pa.) · 22 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Randomized controlled trial (indexed by PubMed)
- Studied in
- People
- Main outcome
- No health outcome
- Intake measured by
- Food provided by researchers
Who paid for it
- Funding
- Independent funding
- Government
- National Institutes of Health
- Government
- National Heart, Lung, and Blood Institute
- Government
- National Cancer Institute
- Government
- National Center for Advancing Translational Sciences
- Government
- NCI NIH HHS
- Government
- NHLBI NIH HHS
- Government
- NIH
- Government
- NHLBI
- Government
- NCATS NIH HHS
- Grants
- National Cancer Institute (P30 CA-077598); National Heart, Lung, and Blood Institute (T32 HL007062); National Heart, Lung, and Blood Institute (5T32HL007062-34); National Center for Advancing Translational Sciences (UL1 TR-000114); National Institutes of Health (P30 CA 77598)
Based on 9 listed funder(s).
Publication
- Published
- 2016-08-18 · Cancer Prev Res (Phila) · vol. 9 · issue 10 · pp. 788–793
- Publisher
- American Association for Cancer Research
- Cited
- 29 citations · more than 88% of similar papers · 2.3× the field average
- References
- 33 works
- Access
- Open access (repository copy)
- Research areas
- Genomics, phytochemicals, and oxidative stress · Bioactive Compounds and Antitumor Agents · Glutathione Transferases and Polymorphisms
- Keywords
- Brassica, Cruciferous vegetables, Urinary system, Urine, Metabolite, Chemistry, Food science, Biomarker, Medicine, Cancer, Biology, Internal medicine, Botany, Biochemistry
- MeSH
- humans, brassica, indoles, glucosinolates, chromatography, liquid, diet, adult, middle aged, tandem mass spectrometry, young adult, biomarkers
10 authors
From US
- Naomi Fujioka · correspondingUniversity of Minnesota
- Benjamin W.S. RansomUniversity of Minnesota
- Steven G. CarmellaUniversity of Minnesota
- Pramod UpadhyayaUniversity of Minnesota
- Bruce R. LindgrenUniversity of Minnesota
- Astia Roper-BatkerUniversity of Vermont
Abstract
Glucobrassicin in Brassica vegetables gives rise to indole-3-carbinol (I3C), a compound with potent anticancer effects in preclinical models. We previously showed that the urinary metabolite 3,3'-diindolylmethane (DIM) could discriminate between volunteers fed high and low doses of Brassica vegetables. However, the quantitative relationship between glucobrassicin exposure and urinary DIM level is unclear. We conducted a clinical trial to examine the hypotheses that a range of glucobrassicin exposure from Brassica vegetables is reflected in urinary DIM and that this effect plateaus. Forty-five subjects consumed vegetables, a mixture of brussels sprouts and/or cabbage, at one of seven discrete dose levels of glucobrassicin ranging from 25 to 500 μmol, once daily for 2 consecutive days. All urine was collected for 24 hours after each vegetable-eating session. Urinary DIM was measured using our published liquid chromatography-electrospray ionization-tandem mass spectrometry-selected reaction monitoring (LC/ESI-MS/MS-SRM) method. Urinary DIM excretion increased predictably with increasing glucobrassicin dose and plateaued between 200 and 300 μmol of glucobrassicin. The association between glucobrassicin dose and urinary DIM was strong and positive (R2 = 0.68). The majority of DIM was excreted in the first 12 hours after vegetable consumption. We conclude that urinary DIM is a reliable biomarker of glucobrassicin exposure and I3C uptake and that feeding glucobrassicin beyond 200 μmol did not consistently lead to more urinary DIM, suggesting a plateau in potential chemopreventive benefit. Cancer Prev Res; 9(10); 788-93. ©2016 AACR.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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