Microwave-assisted extraction, HPLC analysis, and inhibitory effects on carbonic anhydrase I, II, VA, and VII isoforms of 14 blueberry Italian cultivars
Mollica A, Locatelli M, Macedonio G, Carradori S, Sobolev AP, De Salvador RF, Monti SM, Buonanno M, Zengin G, Angeli A, Supuran CT
Journal of enzyme inhibition and medicinal chemistry · 37 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- No health outcome
Who paid for it
- Funding
- Funding not disclosed
Publication
- Published
- 2016-08-10 · J Enzyme Inhib Med Chem · vol. 31 · issue sup4 · pp. 1–6
- Publisher
- Taylor & Francis
- Cited
- 72 citations · more than 98% of similar papers · 7.7× the field average
- Impact
- Top 10% most cited in its field
- References
- 46 works
- Access
- Free to read
- Research areas
- Enzyme function and inhibition · Cholinesterase and Neurodegenerative Diseases · Chemical synthesis and alkaloids
- Keywords
- Chlorogenic acid, Carbonic Anhydrase I, Chemistry, Cultivar, High-performance liquid chromatography, Chromatography, Carbonic anhydrase, Food science, Biochemistry, Enzyme, Botany, Biology
- MeSH
- humans, isoenzymes, carbonic anhydrases, carbonic anhydrase i, carbonic anhydrase ii, plant extracts, carbonic anhydrase inhibitors, chromatography, high pressure liquid, molecular structure, structure-activity relationship, dose-response relationship, drug, microwaves, blueberry plants
11 authors
From IT, TR
- Adriano MollicaUniversity of Chieti-Pescara
- Marcello Locatelli · correspondingUniversity of Chieti-Pescara
- Giorgia MacedonioUniversity of Chieti-Pescara
- Simone CarradoriUniversity of Chieti-Pescara
- Anatoly Petrovich SobolevNational Research Council
- Roberto F. De SalvadorIstituto Sperimentale per la Frutticoltura
Abstract
The multi-component fingerprint and the biological evaluation of plant-derived material are indispensable for the pharmaceutical field, in food quality control procedures, and in all plant-based products. We investigated the quantitative content of biologically active compounds (anthocyanins and chlorogenic acid) of microwave-assisted blueberry extracts from 14 different Italian cultivars, using validated high-performance liquid chromatography-photodiode array detector (HPLC-PDA) method and routinely instrument configuration. The carbonic anhydrase (CA, EC 4.2.1.1) inhibition profiles against several pharmacologically relevant CA isoforms of blueberry extracts and some bioactive compounds were also investigated. The various cultivars showed a highly variable content in anthocyanins and chlorogenic acid, and their CA inhibitory effects were also highly variable. Overall these data prove that antioxidant natural products found in blueberries may be useful for designing pharmacological agents in which various CAs are involved, e.g., antiobesity, antitumor, or anticonvulsants agents.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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